Team:Heidelberg/Parts

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Parts

Measurement Kit

Name Part type Registry link
pSMB_MEASURE: Promoter Measurement plasmid (mammalian) Plasmid backbone Part:BBa_K203100
pSMB_REFERENCE Plasmid Part:BBa_K203099

Core promoters

The following Parts contain the JeT proximal promoter, and a core promoter as described. Core and proximal promoter are separated by a HindIII site. Parts can be applied for testing synthetic proximal promoters. The strength of promoters derived from these parts can be modified by core promoter swapping.

Name Part type Registry link
JeT Regulatory Part:BBa_K203112
JeT proximal-CMV core Regulatory Part:BBa_K203113

Promoters created by RA-PCR

All contain the core promoter of Part:BBa_K203112 and were screened / measured in Part:BBa_K203100.

Name Part type Registry link
Constitutive promoter; 0.4 REU Regulatory Part:BBa_K203110
Constitutive promoter; 1 REU Regulatory Part:BBa_K203118
Constitutive promoter; 2 REU Regulatory Part:BBa_K203111
Constitutive promoter; 2.5 REU Regulatory Part:BBa_K203109
NF-κB regulated promoter Regulatory Part:BBa_K203119
pPARγ regulated promoter 1 Regulatory Part:BBa_K203114
pPARγ regulated promoter 2 Regulatory Part:BBa_K203115

HEARTBEAT Predicted promoters

Name Part type Registry link
SREBP-Responsive, HB1 Regulatory Part:BBa_K203123
SREBP-Responsive, HB3 Regulatory Part:BBa_K203127
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