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Team:Imperial College London/Drylab/Enzyme
From 2009.igem.org
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| - | + | <font face='Calibri' size='5'><b>Kinetics of drug protein of interest</b></font> | |
| - | The protein drug of interest is an enzyme. It will bind to specific substrates and increase the rate of their conversion into products. Therefore, by monitoring either the substrate concentration or the product concentration, we can indirectly see the activity of the enzyme. This is quantified by measuring by enzyme activity in the [https://2009.igem.org/Team:Imperial_College_London/Wetlab/Protocols/cellulase cellulase assay] and [https://2009.igem.org/Team:Imperial_College_London/Wetlab/Protocols/PAH PAH assay]. Enzyme activity is the rate of substrate utilisation /product formation per unit time. | + | Both our proteins of interest, PAH and cellulase, are enzymes to be released in the small intestines, after degradation of the capsule. As part of the study for our system, we would like to calculate the amount of enzyme a capsule must contain so that a PAH-replacement therapy or a grass-based diet becomes a feasible prospect. |
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| + | A necessary step for this calculation is the estimation of the enzymatic activity of our enzymes of interest. | ||
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| + | Rhe standard model for enzymatic action will be used to analyse the raw data (either the substrate concentration or the product concentration over time) gathered in the wetlab. | ||
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| + | <!--The protein drug of interest is an enzyme. It will bind to specific substrates and increase the rate of their conversion into products. Therefore, by monitoring either the substrate concentration or the product concentration, we can indirectly see the activity of the enzyme. This is quantified by measuring by enzyme activity in the [https://2009.igem.org/Team:Imperial_College_London/Wetlab/Protocols/cellulase cellulase assay] and [https://2009.igem.org/Team:Imperial_College_London/Wetlab/Protocols/PAH PAH assay]. Enzyme activity is the rate of substrate utilisation /product formation per unit time. | ||
===Our Goals=== | ===Our Goals=== | ||
Revision as of 15:39, 9 October 2009
- Overview
- The model
- Simulations
Kinetics of drug protein of interest
Both our proteins of interest, PAH and cellulase, are enzymes to be released in the small intestines, after degradation of the capsule. As part of the study for our system, we would like to calculate the amount of enzyme a capsule must contain so that a PAH-replacement therapy or a grass-based diet becomes a feasible prospect.
A necessary step for this calculation is the estimation of the enzymatic activity of our enzymes of interest.
Rhe standard model for enzymatic action will be used to analyse the raw data (either the substrate concentration or the product concentration over time) gathered in the wetlab.
