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Team:Imperial College London/Drylab/Enzyme
From 2009.igem.org
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**Show how the relation between the enzymatic activity of our protein of interest and the rate of breakdown of substrate, d[P]/dt can be exploited. | **Show how the relation between the enzymatic activity of our protein of interest and the rate of breakdown of substrate, d[P]/dt can be exploited. | ||
**Investigate ways to estimate the concentrations of enzyme or substrate if either is unknown as is the case in our experiments. | **Investigate ways to estimate the concentrations of enzyme or substrate if either is unknown as is the case in our experiments. | ||
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<html><a href="https://2009.igem.org/Team:Imperial_College_London/Drylab/Protein_production/Analysis"><img style="vertical-align:bottom;" width=90px align="left" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Learnmore.png"></a></html> about the model assumptions and predictions! | <html><a href="https://2009.igem.org/Team:Imperial_College_London/Drylab/Protein_production/Analysis"><img style="vertical-align:bottom;" width=90px align="left" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Learnmore.png"></a></html> about the model assumptions and predictions! | ||
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===The System=== | ===The System=== | ||
Revision as of 15:48, 9 October 2009
- Overview
- The model
- Simulations
Kinetics of drug protein of interest
Both our proteins of interest, PAH and cellulase, are enzymes to be released in the small intestines, after degradation of the capsule. As part of the study for our system, we would like to calculate the amount of enzyme a capsule must contain so that a PAH-replacement therapy or a grass-based diet becomes a feasible prospect.
A necessary step for this calculation is the estimation of the enzymatic activity of our enzymes of interest.
The standard model for enzymatic action will be used to analyse the raw data (either the substrate concentration or the product concentration over time) gathered in the wetlab.
Contents |
Our Goals
- Overview of system
- Characterise the general dynamics of the system, including its two main modus operandi.
- Assess how its various parameters can affect its output.
- Investigate the validity of the common Michaelis-Menten assumption of enzyme kinetics.
- Data analysis
- Show how the relation between the enzymatic activity of our protein of interest and the rate of breakdown of substrate, d[P]/dt can be exploited.
- Investigate ways to estimate the concentrations of enzyme or substrate if either is unknown as is the case in our experiments.
about the model assumptions and predictions!
The System
Summary of simulation results
Conclusions
References
