Team:Imperial College London/Drylab/Enzyme/Analysis

Assumptions

Enzymatic assumptions:

• The enzyme is specific only for the substrate and not for any other chemicals.
• Only one enzyme, our enzyme of interest is present and participating in the reaction.
• There is negligible formation of product without the enzyme.
• The rate of enzymatic activity remains constant over time because:
  • There is no co-operativity of the system. Binding of substrate to one enzyme binding site doesn't influence the affinity or activity of an adjacent site.
  • No allosteric regulations from either the product or the substrate.
  • There is no product inhibition of the enzyme.

• Total [E] does not change with regards to time. Enzyme will not be used up nor degraded over time, thus [E] at the beginning of the reaction will be the same as [E] at the end of reaction.
• The reaction catalysed is irreversible
• [S] >> [E], such that the free concentration of substrate is very close to the concentration I added. This also ensures a constant substrate concentration throughout the assay. This allows easy determination of [E].
• For which , k1>>k2

Law of mass action assumptions:

• Free diffusion
• Free unrestricted molecular motion

Michaelis-Menten assumption:

• There is a quasi-steady state of [ES] , ie: , during which the enzyme is used for the reaction. This means that the rate of formation of ES complex is equal to the rate of dissociation of ES complex.
• [E]_{0} = [E] + [ES] = constant.
• from the above assumptions, we know that