Team:Imperial College London/Drylab/M1/Protein production/Analysis/Detailed

From 2009.igem.org

Revision as of 15:51, 14 September 2009 by Nuri (Talk | contribs)

In the absence of IPTG

Equation 1: Equation describing the rate of transcription of LacI MRNA (MLacI):
II09 MOD1 M1.jpg

kmlacI is the transcription rate of MlacI (a measure of promoter strength) and dmlacI is the degradation rate. At steady state:

II09 M1 SS1.jpg



Equation 2: Equation describing the rate of translation of LacI protein (PlacI)

II09 MOD1 M1 E2.jpg
At steady state:

II09 M1 SS2.jpg

where klacI is the translation rate of lacI protein and dplacI is the degradation rate of PlacI.Equations 3 and 4 describe the transcription and translation of the protein of interest Pout.


Equation 3: Transcription of Pout

Unlike in the previous case, the output promoter is inducible. In the absence of further information, we model the effect of LacI on transcription/ POPS activity with a Hill function, which represses when amounts are above the threshold K, and activates when PlacI amounts fall below threshold. Such assumption can be revised in the light of contradicting experimental data.

II09 MOD1 M1 E3.jpg

At steady state:

II09 M1 SS3.jpg

When IPTG is introduced

Parameters and values

Mr. Gene   Geneart   Clontech   Giant Microbes