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AFTER THIS: I HAVE ADDED ANOTHER SECTION FOR RESULTS AND DRYLAB, TO STANDARDIZE. | AFTER THIS: I HAVE ADDED ANOTHER SECTION FOR RESULTS AND DRYLAB, TO STANDARDIZE. | ||
===Our results=== | ===Our results=== | ||
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===Enyzme Production=== | ===Enyzme Production=== | ||
Revision as of 18:14, 18 October 2009
Contents |
Module 1: Protein Production
The E.ncapsulator has been designed to produce and deliver polypeptides (amino acid polymers) to the intestine. In order to perform this function successfuly, the polypeptide must be synthesised at a rate that will be sufficient to facilitate its accumulation inside the cell. Once the culture has grown to an optimal level, polypeptide production is chemically induced using IPTG [ref IPTG induction]. With our generic design, it is possible to synthesise any polypeptide. In our project, we have focused on:
- PAH (phenylalanine hydroxilase): Include an explanation
- Cellulase: Include an explanation
- Opiorphin: Include an explanation
Genetic circuit
ADD MORE DETAILS ABOUT GENETIC CIRCUIT AND LITERATURE REVIEW OF GENES. OTHER STUFF HAS MOVED TO DETAILS OF GENETIC CIRCUIT PG. GET CHARLES TO UPLOAD THE GEN.CIRCUIT VIDEO.
This is the genetic circuit for protein production (Module 1).
LacI is produced constitutively by the E. coli bacterium, and it represses the pLambda promoter.
To start the drug manufacturing process, IPTG is pipetted into the system. IPTG will repress LacI. As a result, protein production is de-repressed. Then, the enzymes PAH or cellulase will be produced by the E.ncapsulator.
About the genetic circuit!
Polypeptide Showcase
To demonstrate The E.ncapsulator's versatility, we have chosen to showcase it with both enzymes and peptides. These two classes of polypeptide have very different properties that we have considered and catered for in The E.ncapsulator's design.
About the difference between enzymes and peptides.
AFTER THIS: I HAVE ADDED ANOTHER SECTION FOR RESULTS AND DRYLAB, TO STANDARDIZE.
Our results
Project Tour
Module 1 Contents
