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Team:Imperial College London/M3
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<b>Module 3</b> is the final module of our system. It is designed so that the cell is programmed to destroy it's genetic material after encapsulation has finished. <br><br> | <b>Module 3</b> is the final module of our system. It is designed so that the cell is programmed to destroy it's genetic material after encapsulation has finished. <br><br> | ||
After <b>Module 2</b> has been completed, <i>The E.ncapsulator</i> is programmed to 'genetically self-destruct.' The cell produces enzymes which specifically target and cut DNA sequences, in order to destroy all genetic material contained within the cell. One advantage of using these enzymes for our 'killing' mechanism is that the cell membrane is left intact afterwards, and the protein of interest will still be protected by the encapsulated cell. | After <b>Module 2</b> has been completed, <i>The E.ncapsulator</i> is programmed to 'genetically self-destruct.' The cell produces enzymes which specifically target and cut DNA sequences, in order to destroy all genetic material contained within the cell. One advantage of using these enzymes for our 'killing' mechanism is that the cell membrane is left intact afterwards, and the protein of interest will still be protected by the encapsulated cell. | ||
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| + | More detail can be found [[Imperial_College_London/M3/Detail |here]]. | ||
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==Why== | ==Why== | ||
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As a protective mechanism against DNA destruction due to basal levels of restriction enzyme production, we have made use of the native E. coli Dam methylase protection system. This methylates DNA, which means that only high levels of restriction enzyme (ie. upon trigger) will cleave the DNA. | As a protective mechanism against DNA destruction due to basal levels of restriction enzyme production, we have made use of the native E. coli Dam methylase protection system. This methylates DNA, which means that only high levels of restriction enzyme (ie. upon trigger) will cleave the DNA. | ||
is induced, rendering the bacterium no more than an inanimate shell containing our protein drug of choice. | is induced, rendering the bacterium no more than an inanimate shell containing our protein drug of choice. | ||
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{{Imperial/09/TemplateBottom}} | {{Imperial/09/TemplateBottom}} | ||
Revision as of 12:56, 14 September 2009
Overview
Module 3 is the final module of our system. It is designed so that the cell is programmed to destroy it's genetic material after encapsulation has finished.
After Module 2 has been completed, The E.ncapsulator is programmed to 'genetically self-destruct.' The cell produces enzymes which specifically target and cut DNA sequences, in order to destroy all genetic material contained within the cell. One advantage of using these enzymes for our 'killing' mechanism is that the cell membrane is left intact afterwards, and the protein of interest will still be protected by the encapsulated cell.
More detail can be found here.
