Team:Imperial College London/M3

From 2009.igem.org

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(Overview)
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[[Image:II09_TimelineM3.png| 600px| center]]
[[Image:II09_TimelineM3.png| 600px| center]]
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Module 3 is the final module of the system. This programs the E.ncapsulator to destroy its genetic material after encapsulation has finished.  This prevents any possible pathogenic effects, and also allays health concerns of eating genetically modified organisms. <br>
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[[Image: II09_gmo fears.jpg| right]]
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10th International Symposium on the Biosafety of Genetically Modified Organisms (GM Biosafety Symposium)<br>
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After Module 2 has been completed, genome deletion is triggered by raising the temperature. This is especially suitable since it is difficult for normal chemical induction to penetrate the colanic acid coating. <br>(see [[Team:Imperial_College_London/Temporal_Control/Thermoinduction thermoinduction under temporal control]]) <br>
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[[Image: II09_temp increase.jpg| right]]
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The cell then produces the [[Team:Imperial_College_London/M1/RE restriction enzymes]] DpnII and TaqI which specifically target and cut DNA sequences, in order to destroy all genetic material contained within the cell. <br>
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[[Image: II09_cut dna.jpg| center]]
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To protect against DNA destruction due to basal levels of restriction enzyme production, we have made use of the native E. coli [[Team:Imperial_College_London/M1/Dam Dam methylase protection system]]. This methylates DNA.  Therefore, only high levels of restriction enzyme (ie. after thermal triggering) will cleave the DNA.<br>
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A distinct advantage of using restriction enzymes for our 'killing' mechanism is that the cell membrane is left intact afterwards, and the protein of interest will still be protected by the encapsulated cell.  This renders the bacterium no more than an inanimate shell containing our protein drug of choice. <br>
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[[Image: inanimate shell.jpg| center]]
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<br><br><br><b>Module 3</b> is the final module of our system. It is designed so that the cell is programmed to destroy it's genetic material after encapsulation has finished. <br><br>
<br><br><br><b>Module 3</b> is the final module of our system. It is designed so that the cell is programmed to destroy it's genetic material after encapsulation has finished. <br><br>
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==Why==
==Why==

Revision as of 22:28, 16 September 2009

II09 Thumb m3.pngModule 3 Overview

II09 TimelineM3.png

Module 3 is the final module of the system. This programs the E.ncapsulator to destroy its genetic material after encapsulation has finished. This prevents any possible pathogenic effects, and also allays health concerns of eating genetically modified organisms.

II09 gmo fears.jpg

10th International Symposium on the Biosafety of Genetically Modified Organisms (GM Biosafety Symposium)

After Module 2 has been completed, genome deletion is triggered by raising the temperature. This is especially suitable since it is difficult for normal chemical induction to penetrate the colanic acid coating.
(see Team:Imperial_College_London/Temporal_Control/Thermoinduction thermoinduction under temporal control)

II09 temp increase.jpg

The cell then produces the Team:Imperial_College_London/M1/RE restriction enzymes DpnII and TaqI which specifically target and cut DNA sequences, in order to destroy all genetic material contained within the cell.

II09 cut dna.jpg

To protect against DNA destruction due to basal levels of restriction enzyme production, we have made use of the native E. coli Team:Imperial_College_London/M1/Dam Dam methylase protection system. This methylates DNA. Therefore, only high levels of restriction enzyme (ie. after thermal triggering) will cleave the DNA.

A distinct advantage of using restriction enzymes for our 'killing' mechanism is that the cell membrane is left intact afterwards, and the protein of interest will still be protected by the encapsulated cell. This renders the bacterium no more than an inanimate shell containing our protein drug of choice.

Inanimate shell.jpg


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