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Team:Imperial College London/M3
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Module 3 is the final module of the system. It programs the E.ncapsulator to destroy its genetic material after encapsulation has finished. This prevents any possible pathogenic effects, and also allays health concerns of eating genetically modified organisms. <br> | Module 3 is the final module of the system. It programs the E.ncapsulator to destroy its genetic material after encapsulation has finished. This prevents any possible pathogenic effects, and also allays health concerns of eating genetically modified organisms. <br> | ||
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| + | To learn more about the ethical implications, please click on <i>The E.ncapsulator</i> <html><left><a href="https://2009.igem.org/Team:Imperial_College_London/Stomach"><img width= | ||
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==Killing strategy== | ==Killing strategy== | ||
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[[Image: II09_cut dna.jpg| center]] | [[Image: II09_cut dna.jpg| center]] | ||
To protect against DNA destruction due to basal levels of restriction enzyme production, we have made use of the native E. coli [[Team:Imperial_College_London/M1/Dam| Dam methylase protection system]]. This methylates DNA. Therefore, only high levels of restriction enzyme (ie. after thermal triggering) will cleave the DNA.<br> | To protect against DNA destruction due to basal levels of restriction enzyme production, we have made use of the native E. coli [[Team:Imperial_College_London/M1/Dam| Dam methylase protection system]]. This methylates DNA. Therefore, only high levels of restriction enzyme (ie. after thermal triggering) will cleave the DNA.<br> | ||
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| + | ==Thermoinduction== | ||
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| + | [[Image: II09_temp increase.jpg| right]] | ||
| + | After Module 2 has been completed, genome deletion is triggered by raising the temperature. This is especially suitable since it is difficult for normal chemical induction to penetrate the colanic acid coating. <br> | ||
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| + | The pLambda promoter and cI protein form a thermoregulatable system. <br> | ||
| + | see [[Team:Imperial_College_London/Temporal_Control/Thermoinduction| thermoinduction under temporal control]])<br> | ||
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[[Image:II09_DNApic.png |right|250px]] | [[Image:II09_DNApic.png |right|250px]] | ||
<br><br><br><b>Module 3</b> is the final module of our system. It is designed so that the cell is programmed to destroy it's genetic material after encapsulation has finished. <br><br> | <br><br><br><b>Module 3</b> is the final module of our system. It is designed so that the cell is programmed to destroy it's genetic material after encapsulation has finished. <br><br> | ||
Revision as of 11:13, 18 September 2009
Module 3 Overview
Module 3 is the final module of the system. It programs the E.ncapsulator to destroy its genetic material after encapsulation has finished. This prevents any possible pathogenic effects, and also allays health concerns of eating genetically modified organisms.
To learn more about the ethical implications, please click on The E.ncapsulator 
A distinct advantage of using restriction enzymes for our 'killing' mechanism is that the cell membrane is left intact afterwards, and the protein of interest will still be protected by the encapsulated cell. This renders the bacterium no more than an inanimate shell containing our protein drug of choice.
Killing strategy
After Module 2 has been completed, genome deletion is triggered by raising the temperature.
see thermoinduction under temporal control)
The cell then produces the restriction enzymes DpnII and TaqI. These specifically target and cut short 4 base DNA sequences. As the cutting is frequent, the genetic material contained within the cell will all be destroyed.
To protect against DNA destruction due to basal levels of restriction enzyme production, we have made use of the native E. coli Dam methylase protection system. This methylates DNA. Therefore, only high levels of restriction enzyme (ie. after thermal triggering) will cleave the DNA.
