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Team:Imperial College London/M3/RestrictionEnzymes
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=[[Image:II09_Thumb_m3.png|40px]]<font size='5'><b>Module 3: Genome Deletion Overview</b></font>= | =[[Image:II09_Thumb_m3.png|40px]]<font size='5'><b>Module 3: Genome Deletion Overview</b></font>= | ||
==Restriction Enzymes== | ==Restriction Enzymes== | ||
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In our system, the restriction enzymes DpnII and TaqI are produced. | In our system, the restriction enzymes DpnII and TaqI are produced. | ||
| - | These are 4 base cutters, | + | These restriction enzymes will cleave DNA at recognition sites. This leads to a double-stranded breakage in DNA, which will subsequently result in cell death unless repair is performed in time. |
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| + | These are 4 base cutters, specifically targetting and cutting the sequences GATC and TCGA. With a high frequency of cutting, the repair system would not be able to cope with multiple cleavages, so the genetic material contained within the cell will all be destroyed, including any inserted DNA. <br> | ||
[[Image: II09_cut dna.jpg| center]] | [[Image: II09_cut dna.jpg| center]] | ||
Click here for more information regarding frequency of cutting by 4 cutters | Click here for more information regarding frequency of cutting by 4 cutters | ||
<html><a href="https://2009.igem.org/Team:Imperial_College_London/Restriction_Enzymes"><img style="vertical-align:bottom;" width=90px align="left" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Learnmore.png"></a></html><br><br> about the restriction enzymes TaqI and DpnII. | <html><a href="https://2009.igem.org/Team:Imperial_College_London/Restriction_Enzymes"><img style="vertical-align:bottom;" width=90px align="left" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Learnmore.png"></a></html><br><br> about the restriction enzymes TaqI and DpnII. | ||
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Revision as of 23:35, 6 October 2009
- Overview
- Restriction Enzymes
- Dam Methylation
- Thermoinduction
Module 3: Genome Deletion Overview
Restriction Enzymes
In our system, the restriction enzymes DpnII and TaqI are produced.
These restriction enzymes will cleave DNA at recognition sites. This leads to a double-stranded breakage in DNA, which will subsequently result in cell death unless repair is performed in time.
These are 4 base cutters, specifically targetting and cutting the sequences GATC and TCGA. With a high frequency of cutting, the repair system would not be able to cope with multiple cleavages, so the genetic material contained within the cell will all be destroyed, including any inserted DNA.
Click here for more information regarding frequency of cutting by 4 cutters
about the restriction enzymes TaqI and DpnII.
A distinct advantage of using restriction enzymes for our 'killing' mechanism is that the cell membrane is left intact afterwards, and the protein of interest will still be protected by the encapsulated cell. This renders the bacterium no more than an inanimate shell containing our protein drug of choice.
about the restriction enzymes TaqI and DpnII.
About the ethical implications of live organisms.
