Team:Imperial College London/Major results

From 2009.igem.org

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Wet lab experiments were performed to investigate growth of <i>E. Coli</i> cells under environments of different pH.
Wet lab experiments were performed to investigate growth of <i>E. Coli</i> cells under environments of different pH.
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==<b>Module 3 - Genome Deletion</b>==
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==<b>Module 3</b> - Genome Deletion==
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*The wet lab has also investigated the [[Team:Imperial_College_London/Wetlab/Results/RestrictionDigest | effects of restriction enzymes on cell population]]. Positive results were achieved.
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[[Image:II09_DpnII_Digest.png |400px |left]][[Image:II09_TaqI_Digest.png | 400px |right]]
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From these results, we have shown that restriction enzymes can cleave Dam negative strains, hence killing Dam negative cells. This also proves Dam positive strains are protected again genomic deletion.
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genomic cell death
 
cell death model
cell death model

Revision as of 20:59, 21 October 2009

Contents

Major Results

This page contains highlights of our major results and simulations. It is by no means complete. However, more results will be presented at the jamboree.

Module 2 - Encapsulation

Wet lab experiments were performed to investigate growth of E. Coli cells under environments of different pH.

Module 3 - Genome Deletion

II09 DpnII Digest.png
II09 TaqI Digest.png


From these results, we have shown that restriction enzymes can cleave Dam negative strains, hence killing Dam negative cells. This also proves Dam positive strains are protected again genomic deletion.



cell death model

Module Integration - Autoinduction

crp promoter

diauxic growth

Module Integration - Thermoinduction

hvd gfp


References

[1]Physica A: Statistical and Theoretical Physics, Vol. 188, No. 1-3. (1 September 1992), pp. 404-425. A rigorous derivation of the chemical master Equation

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