Team:Imperial College London/Major results

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(Major Results)
(Simulations: Chemoinduction/Module 1-Output yield of drug of interest)
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==Simulations: Chemoinduction/Module 1-Output yield of drug of interest==
==Simulations: Chemoinduction/Module 1-Output yield of drug of interest==
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Our simulation results indicate that increasing input amounts of IPTG result in a greater yield of polypeptide drug of interest, provided that input concentrations are not within a toxic range. For more information see our [https://2009.igem.org/Team:Imperial_College_London/Drylab/Protein_Production simulation results] and [https://2009.igem.org/Team:Imperial_College_London/Wetlab/Results/Cheminduction/IPTG experimental results on toxicity]. <br>
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Our simulation results indicate that increasing input amounts of IPTG result in a greater yield of polypeptide drug of interest (See [https://2009.igem.org/Team:Imperial_College_London/Drylab/Protein_Production simulation results]). We did an experiment to study how different input concentrations of IPTG affect cell growth, and concluded that it has no significant effect([https://2009.igem.org/Team:Imperial_College_London/Wetlab/Results/Cheminduction IPTG growth curves]). Therefore, we can conclude from the simulations that increasing input amounts of IPTG may lead to increasing amounts of polypeptide drug of interest. <br>
[[Image:II09_SIm_main_prot.jpg]]<br>
[[Image:II09_SIm_main_prot.jpg]]<br>
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Note: Parameters are arbitrary. For a justification see [https://static.igem.org/mediawiki/2009/a/a0/II09_Prot_stability_analysis.pdf the system stability analysis.]
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Note: Parameters used in the system are arbitrary. For a justification see [https://static.igem.org/mediawiki/2009/a/a0/II09_Prot_stability_analysis.pdf the system stability analysis.]
==Experimental: Module 2 - Encapsulation growth curves==
==Experimental: Module 2 - Encapsulation growth curves==

Revision as of 21:12, 20 October 2009

Contents

Major Results

This page contains some, but not all of our major results. Some of the highlights of our simulations and lab data are summarized here. More will be presented at the jamboree.

Simulations: Chemoinduction/Module 1-Output yield of drug of interest

Our simulation results indicate that increasing input amounts of IPTG result in a greater yield of polypeptide drug of interest (See simulation results). We did an experiment to study how different input concentrations of IPTG affect cell growth, and concluded that it has no significant effect(IPTG growth curves). Therefore, we can conclude from the simulations that increasing input amounts of IPTG may lead to increasing amounts of polypeptide drug of interest.
II09 SIm main prot.jpg
Note: Parameters used in the system are arbitrary. For a justification see the system stability analysis.

Experimental: Module 2 - Encapsulation growth curves

Dependent on lab results today

Experimental: Autoinduction - Diauxic growth curve and CRP GFP

Here we can pick a diauxic growth curve and compare directly to a simulation (if I manage to make one work!! :-S)

Simulations: Module 1 - Enzyme kinetics and dosage control

II09 enzymekinetics.png

Many polypeptides of interest are enzymes. This means that detecting how much drug has been produced requires knowledge about their interaction with their respective substrates in the enzymatic assays. Here we assumed that enzymatic activity follows Michaelis-Menten kinetics [7]. This means that we can directly relate enzymatic activity to the required dosage for successful administration of the polypeptide of interest.
Dosage Calculations:
Tianyi upload once we work out activity or whatever is necessary!



Experimental: Module 3 - Thermoinducible promoter

Need Matthieu's latest processed data

Conclusion

Need some inspiration here guys!



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