Team:Imperial College London/Major results

From 2009.igem.org

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Corrected fluorescence of glucose is almost negligible. From this, we have proved the theory that glucose represses the PcstA promoter strongly.  
Corrected fluorescence of glucose is almost negligible. From this, we have proved the theory that glucose represses the PcstA promoter strongly.  
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Also, 10% Casamino Acids in M9 shows the highest corrected fluorescence.
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Also, 10% Casamino Acids in M9 shows the highest corrected fluorescence as well as a high corrected OD600, indicating good cell growth. Therefore, this is our media of choice.
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OD600 at 10% Casamino Acids in M9 is high, indicating good cell growth.
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Revision as of 21:41, 21 October 2009

Contents

Major Results

This page contains highlights of our major results and simulations. It is by no means complete. However, more results will be presented at the jamboree.

Module 2 - Encapsulation

Wet lab experiments were performed to investigate growth of E. Coli cells under environments of different pH.

Module 3 - Genome Deletion

Ii09 111.PNG

The Dam negative strains are fully cleaved at all different concentrations of restriction enzyme. This produces the 'smear' that can be seen, as the DNA fragments are all of varying, but short lengths

From these results, we have shown that restriction enzymes can cleave Dam negative strains, hence killing Dam negative cells. This also proves Dam positive strains are protected again genomic deletion.


  • Furthermore, the dry lab has provided a model on cell population under the production of restriction enzymes.
M3s.6.png

We have shown that an increase in restriction enzyme concentration will decrease total cell population, under the abscence Dam protection.











Module Integration - Autoinduction

  • Experiments were performed on the CRP promoter. From this experiment, we are able to choose the best media for the optimal growth of cells.

Ii09 112.PNG

Corrected fluorescence of glucose is almost negligible. From this, we have proved the theory that glucose represses the PcstA promoter strongly.

Also, 10% Casamino Acids in M9 shows the highest corrected fluorescence as well as a high corrected OD600, indicating good cell growth. Therefore, this is our media of choice.



diauxic growth

Module Integration - Thermoinduction

hvd gfp


References

[1]Physica A: Statistical and Theoretical Physics, Vol. 188, No. 1-3. (1 September 1992), pp. 404-425. A rigorous derivation of the chemical master Equation

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