Latest revision as of 21:02, 13 October 2009

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Cloning Strategy
Protocols
BioBricks
Results
Notebook

Notebook

Here is our daily notebook.

Please update all the experiments that you have performed, and any considerations along the way so that everyone can see the results and others can duplicate your experiments.

September
M	T	W	T	F	S	S
	1	2	3	4	5	6
7	8	9	10	11	12	13
14	15	16	17	18	19	20
21	22	23	24	25	26	27
28	29	30

October
M	T	W	T	F	S	S
			1	2	3	4
5	6	7	8	9	10	11
12	13	14	15	16	17	18
19	20	21	22	23	24	25
26	27	28	29	30	31

BioBrick Progress

The table below shows the progress/status of each BioBrick part/construct:

Key
Submitted
Sequenced
Midi-prepped
Fully verified
Partially verified
Transformed
Ligation pending/Failed
SLIC
Site-directed mutagenesis
Awaiting Mr.Gene

Part Number	Part Name	Status
BBa_K200000	RcsB	Sequenced from mini-prep/SDM on midi-prep
BBa_K200001	Dam methylase	Mini-prepped
BBa_K200002	ygiV (B3023)	Mini-prepped
BBa_K200003	Waal Ligase	Mini-prepped
BBa_K200005	OtsA	Sequenced from midi-prep
BBa_K200006	OtsB	Sequenced from midi-prep
BBa_K200007	Cellulase	Transformed
BBa_K200008	Phenylalanine hydroxylase	Site-directed mutagenesis/mini-preps
BBa_K200009	DpnII restriction enzyme	Awaiting Mr. Gene
BBa_K200009	TaqI restriction enzyme (RBS+TaqI+TT)	Transformed
BBa_K200011	Lambda cI repressor	Awaiting Mr. Gene
BBa_K200012	Lambda promoter	Sequenced from mini-prep
BBa_	pLacI	Sequenced from mini-prep
BBa_	pCstA	Sequenced from mini-prep
BBa_	pCstA+RBS+GFP+TT	Transformed
BBa_	pCstA+RBS+GFP+TT+pLacI+RBS+RFP+TT	Transformed
BBa_	RBS+OtsB	Mini-prepped
BBa_	pCstA+RBS	Mini-prepped
BBa_	LacI+RBS	Mini-prepped

@@ Line 1: / Line 1: @@
 {{Imperial/09/TemplateTop}}
+{{Imperial/09/Tabs/Main/Wetlab/Protocols}}
 =Notebook=
@@ Line 6: / Line 7: @@
 Please update all the experiments that you have performed, and any considerations along the way so that everyone can see the results and others can duplicate your experiments. <br>
 <br>
-{{#Calendar: title=Imperial_College_London/Notebook |year=2009 | month=09}}
+{{#Calendar: title=Imperial_College_London/Notebook |year=2009 | month=09 | left}}{{#Calendar: title=Imperial_College_London/Notebook |year=2009 | month=10 | left}}
 {{Imperial/09/Division}}
-=Progress=
-This table shows our progress with each part:
+=BioBrick Progress=
+The table below shows the progress/status of each BioBrick part/construct:
 {| width="100%" style="background-color:transparent;"
 |
-{| border="2" cellpadding="2" style="text-align:center" align="left" width="30%"
+{| border="2" cellpadding="2" style="text-align:left" align="left" width="30%"
 ! Key
 |- style=background:LimeGreen
@@ Line 22: / Line 24: @@
 | Sequenced
 |- style=background:MediumSlateBlue
-| Midiprepped
+| Midi-prepped
 |- style=background:GreenYellow
 | Fully verified
 |- style=background:MediumVioletRed
-| Partially Verified
+| Partially verified
-|- style=background:LightSeaGreen
+|- style=background:Orchid
 | Transformed
 |- style=background:DarkOrange
@@ Line 34: / Line 36: @@
 | SLIC
 |- style=background:Red
-| Cloning difficulties
+| Site-directed mutagenesis
+|- style=background:YellowGreen
+| Awaiting Mr.Gene
 |}
 |}
-<br>
-<br>
 <br>
 {| border="2" cellpadding="2" style="text-align:left" align="left" width="100%"
 !Part Number !! Part Name !! Status
-|- style=background:LimeGreen
+|- style=background:Red
-|  || || Submitted
+|  BBa_K200000 ||RcsB|| Sequenced from mini-prep/SDM on midi-prep
-|- style=background:GreenYellow
+|- style=background:MediumVioletRed
-|  || || Fully verified
+|  BBa_K200001 ||Dam methylase|| Mini-prepped
+|- style=background:MediumVioletRed
+| BBa_K200002  ||ygiV (B3023)|| Mini-prepped
+|- style=background:MediumVioletRed
+| BBa_K200003  ||Waal Ligase|| Mini-prepped
+|- style=background:MediumSlateBlue
+| BBa_K200005  ||OtsA|| Sequenced from midi-prep
+|- style=background:MediumSlateBlue
+| BBa_K200006  ||OtsB|| Sequenced from midi-prep
+|- style=background:Orchid
+| BBa_K200007  ||Cellulase|| Transformed
+|- style=background:Red
+| BBa_K200008  ||Phenylalanine hydroxylase|| Site-directed mutagenesis/mini-preps
+|- style=background:YellowGreen
+| BBa_K200009  ||DpnII restriction enzyme|| Awaiting Mr. Gene
+|- style=background:Orchid
+| BBa_K200009  ||TaqI restriction enzyme (RBS+TaqI+TT)|| Transformed
+|- style=background:YellowGreen
+| BBa_K200011  ||Lambda cI repressor|| Awaiting Mr. Gene
 |- style=background:DeepSkyBlue
-|  || || Sequenced
+| BBa_K200012  ||Lambda promoter|| Sequenced from mini-prep
+|- style=background:DeepSkyBlue
+| BBa_  ||pLacI|| Sequenced from mini-prep
+|- style=background:DeepSkyBlue
+| BBa_  ||pCstA|| Sequenced from mini-prep
+|- style=background:Orchid
+| BBa_  ||pCstA+RBS+GFP+TT|| Transformed
+|- style=background:Orchid
+| BBa_  ||pCstA+RBS+GFP+TT+pLacI+RBS+RFP+TT|| Transformed
+|- style=background:DarkOrange
+| BBa_  ||RBS+OtsB|| Mini-prepped
+|- style=background:MediumVioletRed
+| BBa_  ||pCstA+RBS|| Mini-prepped
 |- style=background:MediumVioletRed
-|  || || PCR Verified
+| BBa_  ||LacI+RBS|| Mini-prepped
-|- style=background:#cc4400
-|  || ||
-|- style=background:yellow
-|  || || Pending
-|- style=background:maroon
-|  || || Fails to clone
-|- style=background:red
-|  || || Wrong insert cloned
-|- style=background:royalblue
-|  || || Transformed
 |}

Team:Imperial College London/Notebook

From 2009.igem.org

Latest revision as of 21:02, 13 October 2009

Notebook

BioBrick Progress