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Team:Imperial College London/Notebook
From 2009.igem.org
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{{Imperial/09/TemplateTop}} | {{Imperial/09/TemplateTop}} | ||
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=Notebook= | =Notebook= | ||
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Please update all the experiments that you have performed, and any considerations along the way so that everyone can see the results and others can duplicate your experiments. <br> | Please update all the experiments that you have performed, and any considerations along the way so that everyone can see the results and others can duplicate your experiments. <br> | ||
<br> | <br> | ||
| - | {{#Calendar: title=Imperial_College_London/Notebook |year=2009 | month=09}} | + | {{#Calendar: title=Imperial_College_London/Notebook |year=2009 | month=09 | left}}{{#Calendar: title=Imperial_College_London/Notebook |year=2009 | month=10 | left}} |
{{Imperial/09/Division}} | {{Imperial/09/Division}} | ||
| - | =Progress= | + | |
| + | =BioBrick Progress= | ||
The table below shows the progress/status of each BioBrick part/construct: | The table below shows the progress/status of each BioBrick part/construct: | ||
| Line 27: | Line 29: | ||
|- style=background:MediumVioletRed | |- style=background:MediumVioletRed | ||
| Partially verified | | Partially verified | ||
| - | |- style=background: | + | |- style=background:Orchid |
| Transformed | | Transformed | ||
|- style=background:DarkOrange | |- style=background:DarkOrange | ||
| Line 35: | Line 37: | ||
|- style=background:Red | |- style=background:Red | ||
| Site-directed mutagenesis | | Site-directed mutagenesis | ||
| - | |- style=background: | + | |- style=background:YellowGreen |
| Awaiting Mr.Gene | | Awaiting Mr.Gene | ||
|} | |} | ||
| Line 42: | Line 44: | ||
{| border="2" cellpadding="2" style="text-align:left" align="left" width="100%" | {| border="2" cellpadding="2" style="text-align:left" align="left" width="100%" | ||
!Part Number !! Part Name !! Status | !Part Number !! Part Name !! Status | ||
| - | |- style=background: | + | |- style=background:Red |
| - | | BBa_K200000 ||RcsB|| Sequenced | + | | BBa_K200000 ||RcsB|| Sequenced from mini-prep/SDM on midi-prep |
|- style=background:MediumVioletRed | |- style=background:MediumVioletRed | ||
| BBa_K200001 ||Dam methylase|| Mini-prepped | | BBa_K200001 ||Dam methylase|| Mini-prepped | ||
| Line 51: | Line 53: | ||
| BBa_K200003 ||Waal Ligase|| Mini-prepped | | BBa_K200003 ||Waal Ligase|| Mini-prepped | ||
|- style=background:MediumSlateBlue | |- style=background:MediumSlateBlue | ||
| - | | BBa_K200005 ||OtsA|| Sequenced | + | | BBa_K200005 ||OtsA|| Sequenced from midi-prep |
|- style=background:MediumSlateBlue | |- style=background:MediumSlateBlue | ||
| - | | BBa_K200006 ||OtsB|| Sequenced | + | | BBa_K200006 ||OtsB|| Sequenced from midi-prep |
| - | |- style=background: | + | |- style=background:Orchid |
| BBa_K200007 ||Cellulase|| Transformed | | BBa_K200007 ||Cellulase|| Transformed | ||
|- style=background:Red | |- style=background:Red | ||
| - | | BBa_K200008 ||Phenylalanine hydroxylase|| Site-directed mutagenesis/ | + | | BBa_K200008 ||Phenylalanine hydroxylase|| Site-directed mutagenesis/mini-preps |
| - | |- style=background: | + | |- style=background:YellowGreen |
| - | | BBa_K200009 ||DpnII restriction enzyme|| | + | | BBa_K200009 ||DpnII restriction enzyme|| Awaiting Mr. Gene |
| - | |- style=background: | + | |- style=background:Orchid |
| - | | BBa_K200009 ||TaqI restriction enzyme|| | + | | BBa_K200009 ||TaqI restriction enzyme (RBS+TaqI+TT)|| Transformed |
| - | |- style=background: | + | |- style=background:YellowGreen |
| BBa_K200011 ||Lambda cI repressor|| Awaiting Mr. Gene | | BBa_K200011 ||Lambda cI repressor|| Awaiting Mr. Gene | ||
|- style=background:DeepSkyBlue | |- style=background:DeepSkyBlue | ||
| - | | BBa_K200012 ||Lambda promoter|| Sequenced | + | | BBa_K200012 ||Lambda promoter|| Sequenced from mini-prep |
|- style=background:DeepSkyBlue | |- style=background:DeepSkyBlue | ||
| - | | BBa_ ||pLacI|| Sequenced | + | | BBa_ ||pLacI|| Sequenced from mini-prep |
|- style=background:DeepSkyBlue | |- style=background:DeepSkyBlue | ||
| - | | BBa_ ||pCstA|| Sequenced | + | | BBa_ ||pCstA|| Sequenced from mini-prep |
| - | |- style=background: | + | |- style=background:Orchid |
| - | | BBa_ ||pCstA+RBS+ | + | | BBa_ ||pCstA+RBS+GFP+TT|| Transformed |
| + | |- style=background:Orchid | ||
| + | | BBa_ ||pCstA+RBS+GFP+TT+pLacI+RBS+RFP+TT|| Transformed | ||
| + | |- style=background:DarkOrange | ||
| + | | BBa_ ||RBS+OtsB|| Mini-prepped | ||
| + | |- style=background:MediumVioletRed | ||
| + | | BBa_ ||pCstA+RBS|| Mini-prepped | ||
| + | |- style=background:MediumVioletRed | ||
| + | | BBa_ ||LacI+RBS|| Mini-prepped | ||
|} | |} | ||
Latest revision as of 21:02, 13 October 2009
- Back to Hub
- Cloning Strategy
- Protocols
- BioBricks
- Results
- Notebook
Notebook
Here is our daily notebook.
Please update all the experiments that you have performed, and any considerations along the way so that everyone can see the results and others can duplicate your experiments.
| September | ||||||
| M | T | W | T | F | S | S |
| 1 | 2 | 3 | 4 | 5 | 6 | |
| 7 | 8 | 9 | 10 | 11 | 12 | 13 |
| 14 | 15 | 16 | 17 | 18 | 19 | 20 |
| 21 | 22 | 23 | 24 | 25 | 26 | 27 |
| 28 | 29 | 30 | ||||
| October | ||||||
| M | T | W | T | F | S | S |
| 1 | 2 | 3 | 4 | |||
| 5 | 6 | 7 | 8 | 9 | 10 | 11 |
| 12 | 13 | 14 | 15 | 16 | 17 | 18 |
| 19 | 20 | 21 | 22 | 23 | 24 | 25 |
| 26 | 27 | 28 | 29 | 30 | 31 | |
BioBrick Progress
The table below shows the progress/status of each BioBrick part/construct:
|
| Part Number | Part Name | Status |
|---|---|---|
| BBa_K200000 | RcsB | Sequenced from mini-prep/SDM on midi-prep |
| BBa_K200001 | Dam methylase | Mini-prepped |
| BBa_K200002 | ygiV (B3023) | Mini-prepped |
| BBa_K200003 | Waal Ligase | Mini-prepped |
| BBa_K200005 | OtsA | Sequenced from midi-prep |
| BBa_K200006 | OtsB | Sequenced from midi-prep |
| BBa_K200007 | Cellulase | Transformed |
| BBa_K200008 | Phenylalanine hydroxylase | Site-directed mutagenesis/mini-preps |
| BBa_K200009 | DpnII restriction enzyme | Awaiting Mr. Gene |
| BBa_K200009 | TaqI restriction enzyme (RBS+TaqI+TT) | Transformed |
| BBa_K200011 | Lambda cI repressor | Awaiting Mr. Gene |
| BBa_K200012 | Lambda promoter | Sequenced from mini-prep |
| BBa_ | pLacI | Sequenced from mini-prep |
| BBa_ | pCstA | Sequenced from mini-prep |
| BBa_ | pCstA+RBS+GFP+TT | Transformed |
| BBa_ | pCstA+RBS+GFP+TT+pLacI+RBS+RFP+TT | Transformed |
| BBa_ | RBS+OtsB | Mini-prepped |
| BBa_ | pCstA+RBS | Mini-prepped |
| BBa_ | LacI+RBS | Mini-prepped |
