Team:Imperial College London/Temporal Control
From 2009.igem.org
(Difference between revisions)
(→Chemical induction) |
(→Introduction) |
||
Line 12: | Line 12: | ||
== Chemical induction == | == Chemical induction == | ||
+ | <!--[WHAT IS IPTG? HOW DOES IT ACT?]--> | ||
From the [https://2009.igem.org/Team:Imperial_College_London/M1/Genetic Module 1 genetic circuit], in the absence of IPTG in the system, the LacI repressor inhibits production of the protein of interest. | From the [https://2009.igem.org/Team:Imperial_College_London/M1/Genetic Module 1 genetic circuit], in the absence of IPTG in the system, the LacI repressor inhibits production of the protein of interest. | ||
When IPTG is added in: | When IPTG is added in: |
Revision as of 20:44, 16 September 2009
Contents |
Introduction
In the E.ncapsulator, temporal control is an integral part of the design. The 3 modules can only perform their function in this system if they have a temporal mechanism triggering them in a sequential manner. We have achieved this using 3 types of control:
- [Chemical induction]: To kickstart protein drug production (Module1).
- [Autoinduction]: To kickstart Encapsulation (Module 2).
- [Thermoinduction]: To kickstart Genomic Deletion. (Module 3).
Chemical induction
From the Module 1 genetic circuit, in the absence of IPTG in the system, the LacI repressor inhibits production of the protein of interest. When IPTG is added in:
[For more details of the system, click here- (bits of Charles' literature review), includes info on lac operon and how we have modified it]
Autoinduction
b
Thermoinduction
c