Introduction

In the E.ncapsulator, temporal control is an integral part of the design. The 3 modules can only perform their function in this system if they have a temporal mechanism triggering them in a sequential manner. We have achieved this using 3 types of control:

• [Chemical induction]: To kickstart protein drug production (Module1).
• [Autoinduction]: To kickstart Encapsulation (Module 2).
• [Thermoinduction]: To kickstart Genomic Deletion. (Module 3).

Chemical induction

From the Module 1 genetic circuit, in the absence of IPTG in the system, the LacI repressor inhibits production of the protein of interest. When IPTG is added in:

[For more details of the system, click here- (bits of Charles' literature review), includes info on lac operon and how we have modified it]

Autoinduction

b

Thermoinduction

c