"
Team:Imperial College London/Wetlab/Protocols
From 2009.igem.org
(Difference between revisions)
(→Protein Production) |
(→Calibrations) |
||
| (7 intermediate revisions not shown) | |||
| Line 3: | Line 3: | ||
= Protocols = | = Protocols = | ||
| - | ==[[Team:Imperial College London/Wetlab/Protocols/Cellculture| Cell culturing protocols]]== | + | ==[[Team:Imperial College London/Wetlab/Protocols/Cellculture| Cell culturing/cloning protocols]]== |
* [[Team:Imperial_College_London/Wetlab/Protocols/Miniprep |<b>CC1</b>]]: Miniprep | * [[Team:Imperial_College_London/Wetlab/Protocols/Miniprep |<b>CC1</b>]]: Miniprep | ||
* [[Team:Imperial_College_London/Wetlab/Protocols/Midiprep |<b>CC2</b>]]: Midiprep | * [[Team:Imperial_College_London/Wetlab/Protocols/Midiprep |<b>CC2</b>]]: Midiprep | ||
| Line 9: | Line 9: | ||
* <b>CC4</b>: Ligation | * <b>CC4</b>: Ligation | ||
* <b>CC5</b>: Transformation into BL21 using electrical shock | * <b>CC5</b>: Transformation into BL21 using electrical shock | ||
| - | * <b>CC6</b>: Transformation into Top10 using chemical competence | + | * [[Team:Imperial_College_London/Wetlab/Protocols/Cellculture/TransTop10| <b>CC6</b>]]: Transformation into Top10 using chemical competence |
* [[Team:Imperial_College_London/Wetlab/Protocols/LB_Plates |<b>CC7</b>]]: Making LB plates | * [[Team:Imperial_College_London/Wetlab/Protocols/LB_Plates |<b>CC7</b>]]: Making LB plates | ||
* [[Team:Imperial_College_London/Wetlab/Protocols/Registry_Extraction |<b>CC8</b>]]: Extracting DNA from the registry | * [[Team:Imperial_College_London/Wetlab/Protocols/Registry_Extraction |<b>CC8</b>]]: Extracting DNA from the registry | ||
| Line 15: | Line 15: | ||
* [[Team:Imperial College London/Wetlab/Protocols/PCR |<b>CC10</b>]]: PCR | * [[Team:Imperial College London/Wetlab/Protocols/PCR |<b>CC10</b>]]: PCR | ||
* <b>[[Team:Team:Imperial College London/Wetlab/Protocols/Cellculture/CC11| CC11]]</b>: SLIC | * <b>[[Team:Team:Imperial College London/Wetlab/Protocols/Cellculture/CC11| CC11]]</b>: SLIC | ||
| - | *<b>[[Team:Team:Imperial College London/Wetlab/Protocols/Cellculture/CC12| CC12]]</b>: Biobricking parts from | + | *<b>[[Team:Team:Imperial College London/Wetlab/Protocols/Cellculture/CC12| CC12]]</b>: Biobricking parts from oligos |
* [[Team:Imperial_College_London/Wetlab/Protocols/GenomePrep |<b>CC13</b>]]: Genome Prep for Restriction Assay | * [[Team:Imperial_College_London/Wetlab/Protocols/GenomePrep |<b>CC13</b>]]: Genome Prep for Restriction Assay | ||
| Line 21: | Line 21: | ||
* [[Team:Imperial_College_London/Wetlab/Protocols/Abs |<b>CA1</b>]]: Optical Density Calibration | * [[Team:Imperial_College_London/Wetlab/Protocols/Abs |<b>CA1</b>]]: Optical Density Calibration | ||
* GFP fluorescence calibration | * GFP fluorescence calibration | ||
| - | ** <b>CA2</b>: External GFP fluorescence | + | ** [[Imperial_College_London/Wetlab/Protocols/FluorEx| <b>CA2</b>]]: External GFP fluorescence |
| - | ** <b>CA3</b>: Intracellular GFP fluorescence | + | ** [[Imperial_College_London/Wetlab/Protocols/FluorIn| <b>CA3</b>]]: Intracellular GFP fluorescence |
==[[Team:Imperial College London/Wetlab/Protocols/PromoterCharacterisation| Promoter Characterisation]]== | ==[[Team:Imperial College London/Wetlab/Protocols/PromoterCharacterisation| Promoter Characterisation]]== | ||
| Line 62: | Line 62: | ||
--> | --> | ||
| - | =Autoinduction assays= | + | <!--=Autoinduction assays= |
| - | + | ||
==IPTG effect on growth== | ==IPTG effect on growth== | ||
| Line 76: | Line 76: | ||
[[Team:Imperial_College_London/Wetlab/Protocols/IPTGgrowth| See the protocol for more details]] | [[Team:Imperial_College_London/Wetlab/Protocols/IPTGgrowth| See the protocol for more details]] | ||
| - | + | ||
==Lac characterisation and IPTG effect on protein production== | ==Lac characterisation and IPTG effect on protein production== | ||
| Line 92: | Line 92: | ||
[[Team:Imperial_College_London/Wetlab/Protocols/IPTG-RFP| See the protocol for more details]] | [[Team:Imperial_College_London/Wetlab/Protocols/IPTG-RFP| See the protocol for more details]] | ||
| - | + | ||
=Encapsulation assays= | =Encapsulation assays= | ||
==Colanic Acid== | ==Colanic Acid== | ||
Latest revision as of 21:05, 20 October 2009
- Back to Hub
- Cloning Strategy
- Protocols
- BioBricks
- Results
- Notebook
Contents |
Protocols
Cell culturing/cloning protocols
- CC1: Miniprep
- CC2: Midiprep
- CC3: Making cells competent
- CC4: Ligation
- CC5: Transformation into BL21 using electrical shock
- CC6: Transformation into Top10 using chemical competence
- CC7: Making LB plates
- CC8: Extracting DNA from the registry
- CC9: Preparation of XL1-Blue electrocompetent cells & XL1-Blue quality control
- CC10: PCR
- CC11: SLIC
- CC12: Biobricking parts from oligos
- CC13: Genome Prep for Restriction Assay
Calibrations
- CA1: Optical Density Calibration
- GFP fluorescence calibration
Promoter Characterisation
- PP1: Lac Promoter Characterisation
Autoinduction
Protein Production
Encapsulation
- EN1: Colanic acid production amount
- EN2: Colanic acid protection from pH
- EN3: Trehalose production
Killing
- KI1: Thermoinduction
- KI2: Restriction Enzyme activity
