Major results are shown in this section; there's a brief description of each and you can click through to find more detail on them. For minor results, please refer to our Notebook.

Module 1

Module 2

Module 3

Genome Deletion

Restriction Enzyme testing in Dam+ve and Dam-ve Strains

An experiment investigating the effects of methylation on the amount of cleavage by varying concentrations of restriction enzymes.

Temporal Control

Chemical induction

Cell growth for different IPTG concentrations

Testing if IPTG toxicity affects cell growth, and consequently, production of protein of interest.
Rationale: IPTG has been shown in the past to have toxic effects on cell growth if administered in large quantities [http://openwetware.org/wiki/IGEM:IMPERIAL/2009/M1/Modelling/Analysis/literatureIPTG (literature review IPTG)]. However, production of the protein of interest will be induced with IPTG and from the Modelling results, output yield of protein of interest will increase with concentration of IPTG, provided that the levels are non-toxic. The results we present here are a study on how IPTG exerts its effects on cellular growth rate, in order to determine if the concentrations we are using have negative effects on the cultures, and consequently, how this will affect output levels of drug of interest.

Autoinduction

Growth in the presence of Glucose only

Testing the effects of glucose concentration on cell growth: This experiment will be linked to diauxic growth.
Rationale: A cell culture in the presence of glucose grows exponentially. Once it uses up its resource, it stops growing and the population density saturates.
Raw data 06/10 Media:II09_rawdata.xls

Growth in the presence of Glucose + Xylose

Testing the effects of different xylose concentrations on cell growth, for a fixed initial glucose concentration: This experiment will be linked to diauxic growth.
Rationale: A cell culture in the presence of glucose grows exponentially. Once it uses up its resource, it stops growing and the population density saturates. However, in the presence of a secondary carbon source, the cells enter a second exponential phase where they grow and saturate once again when the secondary source has been used up.
NOTE: Same raw data file as above!

Thermoinduction

Harvard+GFP results

Testing the effects of temperature on fluorescence output.
Rationale: Genome deletion is repressed at low temperatures, and triggered when the temperature rises. Here, we are testing the p-lambda promoter by attaching GFP to it, in order to show how the fluorescence output varies when the temperature rises. raw data