Team:KULeuven/7 August 2009

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Revision as of 09:04, 4 September 2009

Project progress

Progress of parts

[edit] Blue Light Receptor

[edit] Vanillin Production

Gel extraction of DNA from A1 (sam5) and A2 (sam8) Results of the nanodrop:

Part concentration (ng/μl) 260/280 λ 260/230 λ
A1 (sam5) 3,4 1,80 0,01
A2 (sam8) 3,1 2,66 0,02
  • COMT was grown
  • sam5 and sam8 were ligated
  • ech and fcs were ligated

[edit] Vanillin Receptor

  • Primers for mutagenesis have arrived
  • We used the DNA parts from the second PCR to insert them into a TOPO-vector. The second PCR showed more results so that is why we used these fragments.
  • LBmedium was made with X-gal to select cells afterwards.
  • Plasmids were inserted in competent cells by heatshock and those cells were plated out on the X-gal medium.

[edit] Key/Lock/Anti-Key