Team:KULeuven/Lab/Vanillin Production

From 2009.igem.org

(Difference between revisions)
(New page: =VANILLIN SYNTHESIS= ==Goal== Making vanillin from tyrosine in a five-step pathway. Making vanillin from tyrosine ==requ...)
(required)
Line 7: Line 7:
==required==
==required==
*biobricks:  
*biobricks:  
-
SAM8: BBa_ I742142: coding sequence without RBS
+
    SAM8: BBa_ I742142: coding sequence without RBS
-
SAM5: no number: RBS site + PstI restriction site removed
+
    SAM5: no number: RBS site + PstI restriction site removed
-
COMT: BBa_I742107: coding sequence without RBS
+
    COMT: BBa_I742107: coding sequence without RBS
-
FCS: BBa_I742115: RBS + fcs in pSB1A2  
+
    FCS: BBa_I742115: RBS + fcs in pSB1A2  
-
        ECH: BBa_I742113: RBS + ech in pSB1A2
+
    ECH: BBa_I742113: RBS + ech in pSB1A2
==Where from==
==Where from==

Revision as of 13:32, 24 July 2009

Contents

VANILLIN SYNTHESIS

Goal

Making vanillin from tyrosine in a five-step pathway.

Making vanillin from tyrosine

required

  • biobricks:
   SAM8: BBa_ I742142: coding sequence without RBS
   SAM5: no number: RBS site + PstI restriction site removed
   COMT: BBa_I742107: coding sequence without RBS
   FCS: BBa_I742115: RBS + fcs in pSB1A2 
   ECH: BBa_I742113: RBS + ech in pSB1A2

Where from

  • 1. COMT is available in 2009 spring distribution plate 1 well 19D

DNA is compatible

  • 2. SAM8, SAM5, COMT, FCS, ECH will be sent to us from the French Lab from the university Edinburgh