Team:KULeuven/Notebook/Blue Light Receptor

From 2009.igem.org

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= Week 1: 6 July 2009 - 12 July 2009 =
= Week 1: 6 July 2009 - 12 July 2009 =
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= Week 2: 13 July 2009 - 19 July 2009 =
= Week 2: 13 July 2009 - 19 July 2009 =
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= Week 3: 20 July 2009 - 26 July 2009 =
= Week 3: 20 July 2009 - 26 July 2009 =
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= Week 4: 27 July 2009 - 2 August 2009 =
= Week 4: 27 July 2009 - 2 August 2009 =
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Revision as of 14:02, 27 July 2009


Contents

Week 1: 6 July 2009 - 12 July 2009

Week 2: 13 July 2009 - 19 July 2009

[edit] Monday

  • YcgF Gene, naturally available in 3 E. Coli strains. Can use the promotor of Ycgf gene for expression
  • Probable strain K12
  • Promotor voor YcgF gene:
    • AACAATCCAGGGTAATGGGTGAGGCGAGAGTAAGACGGTAACAGACATATCTTCTTG TGTCTTTCTTTTAATACCAAAACATAACCGTTTCTTTACATTGATAAAAAATGGAAAAAG TTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTT TTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATT CTGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATG
      (source: [1])

[edit] Tuesday

[edit] Wednesday

  • Mail Institut fur Mikrobiologie der Westfalischen, Wilhelms-Universitat Munster for the plasmid [Done]

Week 3: 20 July 2009 - 26 July 2009

[edit] Thursday

  • Primers for the blue light receptor have arrived

[edit] Friday

  • PCR on promoter region in MC4100 E.coli colony using primers and tested on agarose gel

Week 4: 27 July 2009 - 2 August 2009

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