Team:KULeuven/Notebook/Blue Light Receptor

From 2009.igem.org

(Difference between revisions)
Line 2: Line 2:
{{Team:KULeuven/Common/SubMenu_Project}}
{{Team:KULeuven/Common/SubMenu_Project}}
{{Team:KULeuven/Common/EndHeader}}
{{Team:KULeuven/Common/EndHeader}}
 +
__NOTOC__
==13 July==
==13 July==

Revision as of 13:33, 24 July 2009


13 July

  • YCGF Gene, natural available in [http://ecoliwiki.net/colipedia/index.php/ycgF:Gene 3 E. Coli strains]. Can use the promotor of Ycgf gene for expression
  • Probable strain K12
  • Promotor voor YCGF gene:
    • AACAATCCAGGGTAATGGGTGAGGCGAGAGTAAGACGGTAACAGACATATCTTCTTG TGTCTTTCTTTTAATACCAAAACATAACCGTTTCTTTACATTGATAAAAAATGGAAAAAG TTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTT TTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATT CTGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATG (bron: [www.ncbi.nlm.nih.gov])

14 July

  • Primer selection for the promotor [Done]
    • Send mail to Institut for Biologie-Mikrobiologie ([http://www.ncbi.nlm.nih.gov/pubmed/19240136 The BLUF-EAL protein YcgF acts as a direct anti-repressor in a blue-light response of Escherichia coli]) [Done]

15 July

  • Mail Institut fur Mikrobiologie der Westfalischen, Wilhelms-Universitat Munster for the plasmid [Done]

23 July

  • Primers for the blue light receptor have arrived

24 July

  • PCR on primers and tested on agarose gel
Retrieved from "http://2009.igem.org/Team:KULeuven/Notebook/Blue_Light_Receptor"