Team:Kyoto/CiC/Goals

From 2009.igem.org

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(Goals)
(=Subgoal 2 Prepared liposomes which can take in mitochondrial proteins)
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===Subgoal 2 Prepared liposomes which can take in mitochondrial proteins==
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===Subgoal 2 Prepared liposomes which can take in mitochondrial proteins===
Constructing prepared liposomes which can take in mitochondrial proteins from cytoplasm and confirming whether they import proteins in vitro
Constructing prepared liposomes which can take in mitochondrial proteins from cytoplasm and confirming whether they import proteins in vitro

Revision as of 15:53, 20 October 2009

Goals

In this "cells in cells" project, we set three subgoals.

Subgoal 1 Liposomes that can penetrate through cells membrane

Constructing liposomes that can penetrate through cells membrane and confirming whether they get into cells


Subgoal 2 Prepared liposomes which can take in mitochondrial proteins

Constructing prepared liposomes which can take in mitochondrial proteins from cytoplasm and confirming whether they import proteins in vitro


Subgoal 3 Introducing prepared liposomes into host cells

Introducing prepared liposomes into host cells and confirming whether they take in mitochondrial proteins form cytoplasm in vivo

Future Goals

Well-prepared Liposomes

Construction of double membraned liposomes with all important mitochondrial translocases.


Artificial Mitochondrial Genome

Construction of prepared liposome (or well-prepared liposomes) which have DNA in mitochondrial format.

Chloroplasts

Reconstruction of chloroplasts in similar methods.