"
Team:Kyoto/CiC/Goals
From 2009.igem.org
(→Goals) |
(→Goals) |
||
| Line 29: | Line 29: | ||
In this "cells in cells" project, we set three subgoals. | In this "cells in cells" project, we set three subgoals. | ||
| - | + | ===Subgoal 1 --- Liposomes that can penetrate through cell membrane=== | |
Our first subgoal is construction of liposomes that can penetrate through cell membrane and confirming whether they get into cells. | Our first subgoal is construction of liposomes that can penetrate through cell membrane and confirming whether they get into cells. | ||
| Line 35: | Line 35: | ||
| - | + | ===Subgoal 2 --- Prepared liposomes which can take in mitochondrial proteins=== | |
Our second subgoal is construction of prepared liposomes which can take in mitochondrial proteins from cytoplasm and confirming whether they import proteins in vitro. | Our second subgoal is construction of prepared liposomes which can take in mitochondrial proteins from cytoplasm and confirming whether they import proteins in vitro. | ||
| Line 41: | Line 41: | ||
| - | + | ===Subgoal 3 --- Introducing prepared liposomes into host cells=== | |
Our third subgoal is introducing prepared liposomes into host cells and confirming whether they take in mitochondrial proteins form cytoplasm in vivo.This subgoal requires both of subgoals above. | Our third subgoal is introducing prepared liposomes into host cells and confirming whether they take in mitochondrial proteins form cytoplasm in vivo.This subgoal requires both of subgoals above. | ||
Revision as of 19:58, 20 October 2009
- Home
- Cells in Cells
- Goals
Goals
In this "cells in cells" project, we set three subgoals.
Subgoal 1 --- Liposomes that can penetrate through cell membrane
Our first subgoal is construction of liposomes that can penetrate through cell membrane and confirming whether they get into cells.
Subgoal 2 --- Prepared liposomes which can take in mitochondrial proteins
Our second subgoal is construction of prepared liposomes which can take in mitochondrial proteins from cytoplasm and confirming whether they import proteins in vitro.
Subgoal 3 --- Introducing prepared liposomes into host cells
Our third subgoal is introducing prepared liposomes into host cells and confirming whether they take in mitochondrial proteins form cytoplasm in vivo.This subgoal requires both of subgoals above.
Future Goals
Well-prepared Liposomes
Construction of double membraned liposomes with all important mitochondrial translocases.
Artificial Mitochondrial Genome
Construction of prepared liposome (or well-prepared liposomes) which have DNA in mitochondrial format.
Chloroplasts
Reconstruction of chloroplasts in similar methods.
