Team:Kyoto/GSDD/Goals

From 2009.igem.org

(Difference between revisions)
(goals)
m
Line 28: Line 28:
==Goals==
==Goals==
===Ultimate Goal===
===Ultimate Goal===
-
Our ultimate goal is to make a device which enables us to control exact cell’s life (or death) time depending on the number of cell division.
+
Our ultimate goal is to make a device which enables us to control exact cell’s life time (or death time) depending on the number of cell division times.
===Real Goal===
===Real Goal===
-
Real goal is to suggest some possibility of realization of our ultimate goal.
+
Our real goal is to suggest some possibility of realization of our ultimate goal.  
-
This time we will challenge to make prototype parts of our ultimate goal. The parts are called Timer Vector and Bomb Vector. (See details in “Parts Design” page↵) Using these parts, we will run measurement experiments and analyze the result, then we will discuss about the possibility.
+
-
Below are the concrete sub goals.
+
We will challenge to make a “prototype” device of our ultimate goal. First time, we had planned to construct designed two vectors (Timer vector and Bomb vector) and analyze those functions. However, after considering the span of experimental period, we changed our plan and decided to concentrate on the construction and functional analysis of Timer vector system (see details in Mechanism page).
-
#aaaaa
+
 
-
#bbbbb
+
We designed Timer vector to constitutively express eGFP while keeping repeated sequences of LacI binding site and being stabilized. And also we designed constitutive LacI expression vector. (Totally two vectors) After complete the construction, we will move on to the functional measurement of Timer vector system; whether LacI binds to the both ends of repeated sequences and stabilized the linear Timer vector sufficiently and whether Timer vector can behave like natural linear chromosome and roughly count the replication times. (See below box)
-
#ccccc
+
-
#ddddd
+
</div><!-- /#rightcontents -->
</div><!-- /#rightcontents -->

Revision as of 13:50, 15 October 2009

  1. Home
  2. GSDD
  3. Goals

Goals

Ultimate Goal

Our ultimate goal is to make a device which enables us to control exact cell’s life time (or death time) depending on the number of cell division times.

Real Goal

Our real goal is to suggest some possibility of realization of our ultimate goal.

We will challenge to make a “prototype” device of our ultimate goal. First time, we had planned to construct designed two vectors (Timer vector and Bomb vector) and analyze those functions. However, after considering the span of experimental period, we changed our plan and decided to concentrate on the construction and functional analysis of Timer vector system (see details in Mechanism page).

We designed Timer vector to constitutively express eGFP while keeping repeated sequences of LacI binding site and being stabilized. And also we designed constitutive LacI expression vector. (Totally two vectors) After complete the construction, we will move on to the functional measurement of Timer vector system; whether LacI binds to the both ends of repeated sequences and stabilized the linear Timer vector sufficiently and whether Timer vector can behave like natural linear chromosome and roughly count the replication times. (See below box)