Team:Lethbridge

From 2009.igem.org

(Difference between revisions)

Latest revision as of 03:53, 22 October 2009

Project Overview

The issues surrounding energy production are becoming more prominent with increasing environmental concerns and the rising cost of energy. Microbial fuel cells (MFCs) use biological systems to produce an electrical current. Cyanobacteria are organisms which have been studied in MFCs and have been found to create a current, although not highly efficient (Tsujimura et al., 2001).

We wish to increase the efficiency of the cyanobacteria MFC by introducing microcompartments to create a BioBattery. The microcompartments are created by the production of the protein lumazine synthase forms icosahedral capsids. As a proof of principle we will create this system within Escherichia coli and target two different fluorescent proteins within the microcompartment to observe fluorescence resonance energy transfer. Furthermore, we will be exploring a novel method for the mass production of uniform nanoparticles, which is more efficient and cost effective than current methods.

References

Tsujimura, S., Wadano, A., Kano, K., and Ikeda, T., (2001). Photosynthetic bioelectrochemical cell utilizing cyanobacteria and water-generating oxidase. Enzyme and Microbial Technology. 29, 225-231

University of Lethbridge Sponsors

University of Lethbridge Supporters

The University of Lethbridge iGEM Team would like to thank the many individuals who have given us their time, money or support. Including, but not limited to:

The Wieden Lab

The Kothe Lab

The Selinger Lab

The Biochemistry Teaching Lab and coordinators

The University of Lethbridge Student's Union

David Franz

Nathan Puhl

John Thibault

Sebastian Machula

and

Tony Russell

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-		<li><a href="https://2009.igem.org/Team:Lethbridge#Our_Project:_The_BioBattery">Project Overview</a></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge#Our_Project:_The_BioBattery" style="width:90px;">Project Overview</a></li>
 		<li><a href="https://2009.igem.org/Team:Lethbridge#University_of_Lethbridge_Sponsors">Our Sponsors</a></li>
 		</ul></li>
-	<li><a href="https://2009.igem.org/Team:Lethbridge/Team">Team</a></li>
+	<li><a href="https://2009.igem.org/Team:Lethbridge/Team" style="width:3px;">Team</a></li>
-	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge/Project">Project</a>
+	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge/Project" style="width:10px;">Project</a>
-		<ul style="width:96px;">
+		<ul>
 		<li><a href="https://2009.igem.org/Team:Lethbridge/Project#Project_Details">Project Details</a></li>
-		<li><a href="https://2009.igem.org/Team:Lethbridge/Project#Experiments">Experiments</a></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Project#The_Experiments">Experiments</a></li>
 		<li><a href="https://2009.igem.org/Team:Lethbridge/Project#Results">Results</a></li>
 		</ul></li>
-	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge/Ethics">Ethics</a>
+	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge/Ethics" style="width:40px;">Ethics and Safety</a>
-		<ul style="width:87px;">
+		<ul>
-		<li><a href="https://2009.igem.org/Team:Lethbridge/Ethics#Overview">Overview</a></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Ethics#Ethics_and_SynBio" style="width:80px;">Ethics and Synbio</a></li>
 		<li><a href="https://2009.igem.org/Team:Lethbridge/Ethics#The_Survey">The Survey</a></li>
-		<li><a href="https://2009.igem.org/Team:Lethbridge/Ethics#Results">Results</a></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Ethics#Safety">Safety</a></li>
-		<li><a href="https://2009.igem.org/Team:Lethbridge/Ethics#Analysis">Analysis</a></li>
+                </ul></li>
-		</ul></li>
-	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge/Notebook">Notebook</a>
+	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge/Notebook" style="width:3px;">Notebook</a>
-		<ul style="width:111px;">
+		<ul>
 		<li><a href="https://2009.igem.org/Team:Lethbridge/Notebook#Calendar">Calendar</a></li>
 		<li><a href="https://2009.igem.org/Team:Lethbridge/Notebook#May">May</a></li>
@@ Line 242: / Line 262: @@
 		</ul></li>
-	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge/Team_Meetings">Meetings</a>
+	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge/Team_Meetings" style="width:10px;">Meetings</a>
-		<ul style="width:107px;">
+		<ul>
 		<li><a href="https://2009.igem.org/Team:Lethbridge/Team_Meetings#April">April</a></li>
 		<li><a href="https://2009.igem.org/Team:Lethbridge/Team_Meetings#May">May</a></li>
@@ Line 252: / Line 272: @@
 		<li><a href="https://2009.igem.org/Team:Lethbridge/Team_Meetings#September">September</a></li>
 		<li><a href="https://2009.igem.org/Team:Lethbridge/Team_Meetings#October">October</a></li>
-		</ul></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Team_Meetings#Events">Events</a></li>
+                </ul></li>
-	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge/Modeling">Modeling</a>
-        <ul style="width:90px;"
-       <li><a href="https://2009.igem.org/Team:Lethbridge/Modeling#Collaboration">Collaboration</a></li>
-       </ul></li>
-	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge/Parts">Parts</a>
+	<li><a href="https://2009.igem.org/Team:Lethbridge/Modeling" style="width:10px;">Modeling</a></li>
+	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge/Parts" style="width:10px;">Parts</a>
-		<ul style="width:83px;">
+		<ul>
-		<li><a href="https://2009.igem.org/Team:Lethbridge/Parts#Parts_1-10">Parts 1-10</a></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Parts#Favourites">Favourites</a></li>
-		<li><a href="https://2009.igem.org/Team:Lethbridge/Parts#Parts_11-20">Parts 11-20</a></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Parts#Individual_Parts">Individual Parts</a></li>
-		<li><a href="https://2009.igem.org/Team:Lethbridge/Parts#Parts_21-31">Parts 21-31</a></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Parts#Constructs.2FDevices">Constructs</a></li>
-		<li><a href="https://2009.igem.org/Team:Lethbridge/Parts#Intermediate_Parts">Intermediate Parts</a></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Parts#Intermediate_Parts">Intermediates</a></li>
 		</ul></li>
-	<li><a href="https://2009.igem.org/Team:Lethbridge/Criteria">Judging</a></li>
+	<li><a href="https://2009.igem.org/Team:Lethbridge/Collaboration" style="width:25px;">Collaboration</a></li>
+	<li><a href="https://2009.igem.org/Team:Lethbridge/Criteria" style="width:1px;">Judging</a></li>
+	<li><a href="https://2009.igem.org/Team:Lethbridge/videos" style="width:117px;">Extra Information on SynBio</a></li>
 <li class="qmclear">&nbsp;</li></ul>
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 [[Image:Igembanner.jpg|965px|]]
+==Project Overview==
+[[Image:Biobattery.png|right|275px]]
-<html><a href="https://2009.igem.org/Team:Lethbridge" target="_blank"> <img src="https://static.igem.org/mediawiki/2009/d/d9/V_Lethbridge.JPG" width="110" height="110"></a></html>
+The issues surrounding energy production are becoming more prominent with increasing environmental concerns and the rising cost of energy.  Microbial fuel cells (MFCs) use biological systems to produce an electrical current.  Cyanobacteria are organisms which have been studied in MFCs and have been found to create a current, although not highly efficient (Tsujimura et al., 2001).
+We wish to increase the efficiency of the cyanobacteria MFC by introducing microcompartments to create a BioBattery.  The microcompartments are created by the production of the protein lumazine synthase forms icosahedral capsids.   As a proof of principle we will create this system within Escherichia coli and target two different fluorescent proteins within the microcompartment to observe fluorescence resonance energy transfer.  Furthermore, we will be exploring a novel method for the mass production of uniform nanoparticles, which is more efficient and cost effective than current methods.
+'''References'''
-== Our Project: The BioBattery ==
+Tsujimura, S., Wadano, A., Kano, K., and Ikeda, T., (2001). Photosynthetic
+bioelectrochemical cell utilizing cyanobacteria and water-generating
+oxidase. Enzyme and Microbial Technology. 29, 225-231
+==University of Lethbridge Sponsors==
-With increasing concerns surrounding the environment and rising energy costs, many are devoting more time to address the issues surrounding energy production. In the 2009 Canadian Federal Budget, one billion dollars was designated to develop sustainable energy projects. In keeping with this, the University of Lethbridge iGEM Team is working towards creating an efficient sustainable energy source, the BioBattery.
+<html>
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-The BioBattery is based on using photosynthetic pathways to create energy.  The self-sustaining battery will fix carbon dioxide from the atmosphere, provided that it is supplied with water and sunlight. However, simply using these photosynthetic pathways will not create a BioBattery efficient enough to be economically and environmentally applicable. Therefore, the creation of synthetic microcompartments is necessary to increase the energy output of the BioBattery. The microcompartments can contain the photosynthetic factors needed to maximize BioBattery efficiency.
-''
-== University of Lethbridge Sponsors ==
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 <a href="http://www.uleth.ca" target="_blank">
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+<img src="http://i770.photobucket.com/albums/xx343/ulethigem/logo_fiat_143.jpg"></a></td>
+<td>
 <a href="http://www.albertaingenuity.ca/international/genetically/engineered/machines/igem" target="_blank">
-<img src="http://s904.photobucket.com/albums/ac246/MegLand/Alberta1.jpg"></a>
+<img src="http://i770.photobucket.com/albums/xx343/ulethigem/AlbertaIngenuitylogo.jpg"></a></td>
+<td><a href="http://www.rainin.com/canada/default_can.asp" target="_blank">
-<a href="http://mrgene.com/desktopdefault.aspx/tabid-2/" target="_blank">
+<img src="http://i770.photobucket.com/albums/xx343/ulethigem/RAININ_Logo_Japan_big.jpg"></a>
-<img src="http://s904.photobucket.com/albums/ac246/MegLand/Mrgene.jpg"></a>
+</td>
+</tr>
+<tr>
+<td><a href="http://mrgene.com/desktopdefault.aspx/tabid-2/" target="_blank">
+<img src="http://i770.photobucket.com/albums/xx343/ulethigem/logo_mrgene.jpg"></a></td>
+<td>
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 <img src="http://s904.photobucket.com/albums/ac246/MegLand/Viterra.jpg"></a>
+</td>
+<td>
+<a href="http://www.vwrcanlab.com/" target="_blank">
+<img src="http://s904.photobucket.com/albums/ac246/MegLand/VWR.jpg"></a></td>
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+==University of Lethbridge Supporters==
+The University of Lethbridge iGEM Team would like to thank the many individuals who have given us their time, money or support.  Including, but not limited to:
+The Wieden Lab
+The Kothe Lab
+The Selinger Lab
+The Biochemistry Teaching Lab and coordinators
+The University of Lethbridge Student's Union
+David Franz
+Nathan Puhl
+John Thibault
+Sebastian Machula
+and
+Tony Russell