Team:Lethbridge

From 2009.igem.org

(Difference between revisions)

Latest revision as of 03:53, 22 October 2009

Project Overview

The issues surrounding energy production are becoming more prominent with increasing environmental concerns and the rising cost of energy. Microbial fuel cells (MFCs) use biological systems to produce an electrical current. Cyanobacteria are organisms which have been studied in MFCs and have been found to create a current, although not highly efficient (Tsujimura et al., 2001).

We wish to increase the efficiency of the cyanobacteria MFC by introducing microcompartments to create a BioBattery. The microcompartments are created by the production of the protein lumazine synthase forms icosahedral capsids. As a proof of principle we will create this system within Escherichia coli and target two different fluorescent proteins within the microcompartment to observe fluorescence resonance energy transfer. Furthermore, we will be exploring a novel method for the mass production of uniform nanoparticles, which is more efficient and cost effective than current methods.

References

Tsujimura, S., Wadano, A., Kano, K., and Ikeda, T., (2001). Photosynthetic bioelectrochemical cell utilizing cyanobacteria and water-generating oxidase. Enzyme and Microbial Technology. 29, 225-231

University of Lethbridge Sponsors

University of Lethbridge Supporters

The University of Lethbridge iGEM Team would like to thank the many individuals who have given us their time, money or support. Including, but not limited to:

The Wieden Lab

The Kothe Lab

The Selinger Lab

The Biochemistry Teaching Lab and coordinators

The University of Lethbridge Student's Union

David Franz

Nathan Puhl

John Thibault

Sebastian Machula

and

Tony Russell

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-	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge">Home</a>
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-		<ul style="width:59px;">
+		<ul>
-		<li><a href="https://2009.igem.org/Team:Lethbridge#Our_Project:_The_BioBattery">Project Overview</a></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge#Our_Project:_The_BioBattery" style="width:90px;">Project Overview</a></li>
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@@ Line 220: / Line 242: @@
 		</ul></li>
-	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge/Ethics">Ethics</a>
+	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge/Ethics" style="width:40px;">Ethics and Safety</a>
-		<ul style="width:61px;">
+		<ul>
-		<li><a href="https://2009.igem.org/Team:Lethbridge/Ethics#Ethics_and_SynBio">Ethics and Synbio</a></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Ethics#Ethics_and_SynBio" style="width:80px;">Ethics and Synbio</a></li>
 		<li><a href="https://2009.igem.org/Team:Lethbridge/Ethics#The_Survey">The Survey</a></li>
-		</ul></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Ethics#Safety">Safety</a></li>
+                </ul></li>
-	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge/Notebook">Notebook</a>
+	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge/Notebook" style="width:3px;">Notebook</a>
-		<ul style="width:77px;">
+		<ul>
 		<li><a href="https://2009.igem.org/Team:Lethbridge/Notebook#Calendar">Calendar</a></li>
 		<li><a href="https://2009.igem.org/Team:Lethbridge/Notebook#May">May</a></li>
@@ Line 239: / Line 262: @@
 		</ul></li>
-	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge/Team_Meetings">Meetings</a>
+	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge/Team_Meetings" style="width:10px;">Meetings</a>
-		<ul style="width:73px;">
+		<ul>
 		<li><a href="https://2009.igem.org/Team:Lethbridge/Team_Meetings#April">April</a></li>
 		<li><a href="https://2009.igem.org/Team:Lethbridge/Team_Meetings#May">May</a></li>
@@ Line 249: / Line 272: @@
 		<li><a href="https://2009.igem.org/Team:Lethbridge/Team_Meetings#September">September</a></li>
 		<li><a href="https://2009.igem.org/Team:Lethbridge/Team_Meetings#October">October</a></li>
-		</ul></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Team_Meetings#Events">Events</a></li>
+                </ul></li>
-	<li><a href="https://2009.igem.org/Team:Lethbridge/Modeling">Modeling</a></li>
+	<li><a href="https://2009.igem.org/Team:Lethbridge/Modeling" style="width:10px;">Modeling</a></li>
-	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge/Parts">Parts</a>
+	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge/Parts" style="width:10px;">Parts</a>
-		<ul style="width:56px;">
+		<ul>
 		<li><a href="https://2009.igem.org/Team:Lethbridge/Parts#Favourites">Favourites</a></li>
-		<li><a href="https://2009.igem.org/Team:Lethbridge/Parts#Constructs">Constructs</a></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Parts#Individual_Parts">Individual Parts</a></li>
-		<li><a href="https://2009.igem.org/Team:Lethbridge/Parts#Intermediates">Intermediates</a></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Parts#Constructs.2FDevices">Constructs</a></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Parts#Intermediate_Parts">Intermediates</a></li>
 		</ul></li>
-	<li><a href="https://2009.igem.org/Team:Lethbridge/Collaboration">Collaboration</a></li>
+	<li><a href="https://2009.igem.org/Team:Lethbridge/Collaboration" style="width:25px;">Collaboration</a></li>
-	<li><a href="https://2009.igem.org/Team:Lethbridge/Criteria">Judging</a></li>
+	<li><a href="https://2009.igem.org/Team:Lethbridge/Criteria" style="width:1px;">Judging</a></li>
-	<li><a href="https://2009.igem.org/Team:Lethbridge/videos">Extra Information on SynBio</a></li>
+	<li><a href="https://2009.igem.org/Team:Lethbridge/videos" style="width:117px;">Extra Information on SynBio</a></li>
 <li class="qmclear">&nbsp;</li></ul>
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-[[Image:Igembanner.jpg|965px|]]
+[[Image:Igembanner.jpg|965px|]]
 ==Project Overview==
-With increasing concerns surrounding the environment and rising energy costs, many are devoting more time to address the issues surrounding energy production. In the 2009 Canadian Federal Budget, one billion dollars was designated to develop sustainable energy projects. In keeping with this, the University of Lethbridge iGEM Team is working towards creating an efficient sustainable energy source, the BioBattery.
+[[Image:Biobattery.png|right|275px]]
-The BioBattery is based on using photosynthetic pathways to create energy.  The self-sustaining battery will fix carbon dioxide from the atmosphere, provided that it is supplied with water and sunlight. However, simply using these photosynthetic pathways will not create a BioBattery efficient enough to be economically and environmentally applicable. Therefore, the creation of synthetic microcompartments is necessary to increase the energy output of the BioBattery. The microcompartments can contain the photosynthetic factors needed to maximize BioBattery efficiency.
+The issues surrounding energy production are becoming more prominent with increasing environmental concerns and the rising cost of energy.  Microbial fuel cells (MFCs) use biological systems to produce an electrical current.  Cyanobacteria are organisms which have been studied in MFCs and have been found to create a current, although not highly efficient (Tsujimura et al., 2001).
+We wish to increase the efficiency of the cyanobacteria MFC by introducing microcompartments to create a BioBattery.  The microcompartments are created by the production of the protein lumazine synthase forms icosahedral capsids.   As a proof of principle we will create this system within Escherichia coli and target two different fluorescent proteins within the microcompartment to observe fluorescence resonance energy transfer.  Furthermore, we will be exploring a novel method for the mass production of uniform nanoparticles, which is more efficient and cost effective than current methods.
+'''References'''
+Tsujimura, S., Wadano, A., Kano, K., and Ikeda, T., (2001). Photosynthetic
+bioelectrochemical cell utilizing cyanobacteria and water-generating
+oxidase. Enzyme and Microbial Technology. 29, 225-231
 ==University of Lethbridge Sponsors==
@@ Line 285: / Line 321: @@
 <body>
-<table width="300" border="0.5">
+<table width="965" border="0.5">
   <tr>
 <td>
 <a href="http://www.uleth.ca" target="_blank">
-<img src="http://www.crps.ab.ca/crpsold/bchs/logo_fiat_143.jpg"></a></td>
+<img src="http://i770.photobucket.com/albums/xx343/ulethigem/logo_fiat_143.jpg"></a></td>
 <td>
 <a href="http://www.albertaingenuity.ca/international/genetically/engineered/machines/igem" target="_blank">
-<img src="http://www.stmu.ab.ca/Discovering%20Science/images/AlbertaIngenuitylogo.jpg"></a></td>
+<img src="http://i770.photobucket.com/albums/xx343/ulethigem/AlbertaIngenuitylogo.jpg"></a></td>
+<td><a href="http://www.rainin.com/canada/default_can.asp" target="_blank">
+<img src="http://i770.photobucket.com/albums/xx343/ulethigem/RAININ_Logo_Japan_big.jpg"></a>
+</td>
 </tr>
 <tr>
 <td><a href="http://mrgene.com/desktopdefault.aspx/tabid-2/" target="_blank">
-<img src="http://s904.photobucket.com/albums/ac246/MegLand/Mrgene.jpg"></a></td>
+<img src="http://i770.photobucket.com/albums/xx343/ulethigem/logo_mrgene.jpg"></a></td>
 <td>
 <a href="http://www.viterra.ca/portal/wps/portal/viterra" target="_blank">
 <img src="http://s904.photobucket.com/albums/ac246/MegLand/Viterra.jpg"></a>
-</td>
-</tr>
-<tr>
-<td><a href="http://www.rainin.com/canada/default_can.asp" target="_blank">
-<img src="http://s904.photobucket.com/albums/ac246/MegLand/Rainin1.jpg"></a>
 </td>
 <td>
@@ Line 311: / Line 345: @@
 </tr>
 </table>
 </body>
 </html>
+<html>
+<head>
 <html>
@@ Line 394: / Line 435: @@
 		border-width:1px;
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+		border-color:#3C13AF;
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@@ Line 403: / Line 444: @@
 		padding:3px 5px 3px 5px;
 		background-color:transparent;
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+		color:#230672;
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+		border-color:#230672;
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@@ Line 415: / Line 456: @@
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+		background-color:#476DD5;
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@@ Line 424: / Line 465: @@
 	#qm0 ul li:hover>a
 	{
-		background-color:#666666;
+		background-color:#476DD5;
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@@ Line 440: / Line 481: @@
 	body #qm0 div .qmactive, body #qm0 div .qmactive:hover
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@@ Line 478: / Line 519: @@
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@@ Line 509: / Line 549: @@
 </head>
 <body>
 <ul id="qm0" class="qmmc">
-	<li><a href="https://2009.igem.org/Team:Lethbridge/Team">Home</a></li>
+	<li><a href="https://2009.igem.org/Team:Lethbridge">Home</a></li>
-	<li><a href="https://2009.igem.org/Team:Lethbridge/Team">Top of Page</a></li>
+	<li><a href="https://2009.igem.org/Team:Lethbridge">Top of Page</a></li>
 <li class="qmclear">&nbsp;</li></ul>
@@ Line 521: / Line 560: @@
 </body>
 </html>
+==University of Lethbridge Supporters==
+The University of Lethbridge iGEM Team would like to thank the many individuals who have given us their time, money or support.  Including, but not limited to:
+The Wieden Lab
+The Kothe Lab
+The Selinger Lab
+The Biochemistry Teaching Lab and coordinators
+The University of Lethbridge Student's Union
+David Franz
+Nathan Puhl
+John Thibault
+Sebastian Machula
+and
+Tony Russell