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Team:NTU-Singapore/Project/Prototype
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In the [[Team:NTU-Singapore/Project/System | previous section]], we have covered the systemized our objectives for use in a biological ''machine''. We have also further broken the system down into specific devices. | In the [[Team:NTU-Singapore/Project/System | previous section]], we have covered the systemized our objectives for use in a biological ''machine''. We have also further broken the system down into specific devices. | ||
| - | Due to time and equipment constraints, we will not detail the design of the ideal system, since we will not be able to build it anyway. Instead, we will focus on designing a full-blown prototype system as a proof-of-concept. | + | Due to time and equipment constraints, we will not detail the design of the ideal system, since we will not be able to build it anyway. Instead, we will focus on designing a full-blown prototype system as a proof-of-concept, using E.Coli as our model organism.<br class="clear" /> |
| + | == Prototype Design == | ||
| + | Recall the construct layout for the ideal system. | ||
| + | [[Image:NTUsysdev.gif|center]] | ||
| + | |||
| + | Our prototype will utilize a similar layout. We will detail the '''parts''' we have identified for our devices. | ||
| + | |||
| + | |||
| + | '''Sense device''' : | ||
| + | |||
| + | * Arguably the most important gene sequence, we have decided to use the NO-sensitive promoter region of the NorV/W gene sequence endogeneous in E.Coli. | ||
| + | ** This NO-sensitive promoter (we will refer to it as pNO henceforth) | ||
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Revision as of 03:29, 19 October 2009
Our Prototype
Contents |
In the previous section, we have covered the systemized our objectives for use in a biological machine. We have also further broken the system down into specific devices.
Due to time and equipment constraints, we will not detail the design of the ideal system, since we will not be able to build it anyway. Instead, we will focus on designing a full-blown prototype system as a proof-of-concept, using E.Coli as our model organism.
Prototype Design
Recall the construct layout for the ideal system.
Our prototype will utilize a similar layout. We will detail the parts we have identified for our devices.
Sense device :
- Arguably the most important gene sequence, we have decided to use the NO-sensitive promoter region of the NorV/W gene sequence endogeneous in E.Coli.
- This NO-sensitive promoter (we will refer to it as pNO henceforth)
