Team:NTU-Singapore/Project/Wetlab

Conclusive Results

Successful Constructs

The following are the constructs that we have shown to be working.

1. J23119-B0034-NorR-B0015 (system construct)
2. J23119-B0034-GFP-B0015 (characterization construct)
3. pLacI-B0034-CHE-B0015 (test construct)
4. J23199-B0034-HO1-B0015-J23119-B0034-IFP1.4-B0015 (test construct)

The first construct is a system construct, in that it was intended for our system to feature this as a functional part.

The second construct is a characterization construct to characterize the modular function of the J23119 promoter in the transcription of NorR.

The third construct is also a test construct to only test and characterize the enzymatic activity of CHE.

The fourth construct is a test construct to only test the reporting ability of IFP.