Team:Osaka/Brainstorming2

From 2009.igem.org

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=07/10/2009 Paint and Illumination =
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Brainstorming with one brain that belongs to nori
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==Brainstorming with one brain==
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=Operation=
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==Operation==
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1)Cell to Cell communication (Quorum sensing)
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===Cell to Cell communication (Quorum sensing)===
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*orthogonal cell-cell signaling idealy...
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*orthogonal cell-cell signaling will be required for our ART painting
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Existing systems:
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Existing systems->
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*LuxI-LuxR
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*LuxI-LuxR  
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*LasI-LasR
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*ciniI-cinR
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*RhII-RhIR
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*Evaluate diffuse of signals - PoPs, RiPs and Degradation
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===Timer or counter(optional addon)===
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*well known genetic circuit
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*light or sound induced?
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==Output -pattern and painting- ==
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===patterning===
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we have to control the motility of E.coli or salmonella on soft-agar plate
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===Stop rotation of flagella motor===
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*use molecular clutch (iGEM08 Imperial college) [[http://partsregistry.org/partsdb/get_part.cgi?part=BBa_K143032]]
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->However, this parts was characterized only in butilus.
 +
 
 +
===Stop viased random walk===
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express che?
 +
 
 +
===Stop flagellar assembly===
 +
*use the strain that lacks flagella gene. But Inactivating auto inducer will be needed!
 +
 
 +
 
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==Art Challange==
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*no team had done this theme. Ethics and environmental issue was emphasized.

Latest revision as of 20:11, 29 July 2009

Brainstorming with one brain that belongs to nori

Contents

Operation

Cell to Cell communication (Quorum sensing)

  • orthogonal cell-cell signaling will be required for our ART painting

Existing systems->

  • LuxI-LuxR
  • LasI-LasR
  • ciniI-cinR
  • RhII-RhIR
  • Evaluate diffuse of signals - PoPs, RiPs and Degradation

Timer or counter(optional addon)

  • well known genetic circuit
  • light or sound induced?

Output -pattern and painting-

patterning

we have to control the motility of E.coli or salmonella on soft-agar plate

Stop rotation of flagella motor

  • use molecular clutch (iGEM08 Imperial college) [[1]]

->However, this parts was characterized only in butilus.

Stop viased random walk

express che?

Stop flagellar assembly

  • use the strain that lacks flagella gene. But Inactivating auto inducer will be needed!


Art Challange

  • no team had done this theme. Ethics and environmental issue was emphasized.