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Team:Osaka/MOTILITY
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| - | Motile cells, such as <i>Escherichia Coli</i> and <I>Salmonella typhimurium</I> swim by means of flagella. So, we can regard cells as biological paints, we named ColorColi, that can move automatically. Although we can create a lot of artworks (you can see our works at <a href="https://2009.igem.org/Team:Osaka/WORKS">WORKS</a>) by using innate motility of cells, engineering cells motility should expand the application of ColorColi in Art. This year, we aimed to stop the swarming motility of cell for out-put of arithmetic processing by cell-cell communication.(see <a href="https://2009.igem.org/Team:Osaka/SIGNAl">SIGNAL</a>). < | + | Motile cells, such as <i>Escherichia Coli</i> and <I>Salmonella typhimurium</I> swim by means of flagella. So, we can regard cells as biological paints, we named ColorColi, that can move automatically. Although we can create a lot of artworks (you can see our works at <a href="https://2009.igem.org/Team:Osaka/WORKS">WORKS</a>) by using innate motility of cells, engineering cells motility should expand the application of ColorColi in Art. This year, we aimed to stop the swarming motility of cell for out-put of arithmetic processing by cell-cell communication.(see <a href="https://2009.igem.org/Team:Osaka/SIGNAl">SIGNAL</a>). </p> |
| - | + | <p>In past iGEM projects, several teams tried to control cell motility (リンク iHKU2008, Imperial2008, Penn State Uni2006). However, their approaches require modified or specified chassis. <br> | |
So we made a new part to inhibit flagellar assembly and as a result stop the motility and tested the compatibility of <a href="http://partsregistry.org/Part:BBa_K143032">EpsE</a> that work as molecular clutch and stop the motor rotation. | So we made a new part to inhibit flagellar assembly and as a result stop the motility and tested the compatibility of <a href="http://partsregistry.org/Part:BBa_K143032">EpsE</a> that work as molecular clutch and stop the motor rotation. | ||
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| - | <p>Bacterial flagellar assembly is proceed highly coordinating manner<a href="#link1">[1]</a>. </p> | + | <p>Bacterial flagellar assembly is proceed highly coordinating manner<a href="#link1">[1]</a>. </p> <br> |
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Revision as of 12:07, 10 October 2009
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MOTILITY
Overview
Under construction
Object
Motile cells, such as Escherichia Coli and Salmonella typhimurium swim by means of flagella. So, we can regard cells as biological paints, we named ColorColi, that can move automatically. Although we can create a lot of artworks (you can see our works at WORKS) by using innate motility of cells, engineering cells motility should expand the application of ColorColi in Art. This year, we aimed to stop the swarming motility of cell for out-put of arithmetic processing by cell-cell communication.(see SIGNAL).
In past iGEM projects, several teams tried to control cell motility (リンク iHKU2008, Imperial2008, Penn State Uni2006). However, their approaches require modified or specified chassis.
So we made a new part to inhibit flagellar assembly and as a result stop the motility and tested the compatibility of EpsE that work as molecular clutch and stop the motor rotation.
Design
Bacterial flagellar assembly is proceed highly coordinating manner[1].
