Team:PKU Beijing

From 2009.igem.org

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<span style="font-family: Arial; font-size: 18pt">Project Summary</span><hr>
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'''A Genetic Circuit for Directed Evolution ''in vivo'''''
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Directed evolution is a powerful tool for answering scientific questions or constructing novel biological systems. Here we present a simple genetic circuit for ''in vivo'' directed evolution which comprises minimal elements for random mutation and artificial selection. We engineer yeast to generate the DNA mutator hAID, an essential protein in adaptive immunity, and target it specifically to a gene of interest. The target gene will be mutated and consequently promptly evolves. By linking the expression of hAID repressor LacI and favorite gene functionality, the mutation rate inversely correlates between the functionality of the desired gene and hAID. This circuit may be adopted for ''in vivo'' evolution in eukaryotic system on genetically encoded targets. It has a variety of potential applications in academic and industrial contexts, theoretically most inter-molecular interaction that involves proteins and RNAs. <br>
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[https://2008.igem.org/Team:Peking_University/Project Learn More...]
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      <p>Project Summary</p>
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      <p><strong>A Genetic Circuit for Directed Evolution <em>in vivo</em> </strong></p>
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      <p>Directed evolution is a powerful tool for answering scientific questions or   constructing novel biological systems. Here we present a simple genetic circuit   for in vivo directed evolution which comprises minimal elements for random   mutation and artificial selection. We engineer yeast to generate the DNA mutator   hAID, an essential protein in adaptive immunity, and target it specifically to a   gene of interest. The target gene will be mutated and consequently promptly   evolves. By linking the expression of hAID repressor LacI and favorite gene   functionality, the mutation rate inversely correlates between the functionality   of the desired gene and hAID. This circuit may be adopted for in vivo evolution   in eukaryotic system on genetically encoded targets. It has a variety of   potential applications in academic and industrial contexts, theoretically most   inter-molecular interaction that involves proteins and RNAs. Learn More...</p>
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      <p>Peking University<br />
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      <p>Peking University is a comprehensive and National key university. The campus,  known as &quot;Yan Yuan&quot;-- the gardens of Yan, is situated at the northeast of the  Haidian District at the western suburbs of Beijing. It stands near the Yuan Ming  Gardens and the Summer Palace. </p>
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      <p>The university has made an effective combination of the research on important  scientific issues with the training of personnel with high level specialized  knowledge and professional skill as demanded by the country's socialist  modernization. It strives not only for the simultaneous improvements in teaching  and research work, but also for the promotion of interaction and mutual  promotion among various subjects. </p>
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      <p>Peking University has become a center for teaching and research and a  university of the new type, consisting of diverse branches of learning such as  pure and applied sciences, social sciences and the humanities, and sciences of  management and education. Its aim is to rank among the world's best universities  at the beginning of the next century. Learn More... </p>
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      <p>Road to Jamboree</p>
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      <p>Acknowledgement</p>
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<p>&nbsp;</p>
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<span style="font-family: Arial; font-size: 18pt">Peking University</span><hr>
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:Peking University is a comprehensive and National key university. The campus, known as "Yan Yuan"-- the gardens of Yan, is situated at the  northeast of the Haidian District at the western suburbs of Beijing. It stands near the Yuan Ming Gardens and the Summer Palace.
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<br>
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:The university has made an effective combination of the research on important scientific  issues with the training of personnel with high level specialized knowledge and professional skill as demanded by the country's socialist modernization. It strives not only for the  simultaneous improvements in teaching and research work, but also for the promotion of  interaction and mutual promotion among various subjects.
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:Peking University has become a center for teaching and research and a university of the new type, consisting  of diverse branches of learning such as pure and applied sciences, social sciences and the  humanities, and sciences of management and education. Its aim is to rank among the world's  best universities at the beginning of the next century.
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:[http://en.pku.edu.cn/ Learn More...]
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:[https://2008.igem.org/Team:Peking_University/Team Learn More...]
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<span style="font-family: Arial; font-size: 18pt">Acknowledgement</span><hr>
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Revision as of 19:31, 23 June 2009

iGEM09 PKU_Beijing

 

 

  
   
 

 
 

Project Summary

A Genetic Circuit for Directed Evolution in vivo

Directed evolution is a powerful tool for answering scientific questions or constructing novel biological systems. Here we present a simple genetic circuit for in vivo directed evolution which comprises minimal elements for random mutation and artificial selection. We engineer yeast to generate the DNA mutator hAID, an essential protein in adaptive immunity, and target it specifically to a gene of interest. The target gene will be mutated and consequently promptly evolves. By linking the expression of hAID repressor LacI and favorite gene functionality, the mutation rate inversely correlates between the functionality of the desired gene and hAID. This circuit may be adopted for in vivo evolution in eukaryotic system on genetically encoded targets. It has a variety of potential applications in academic and industrial contexts, theoretically most inter-molecular interaction that involves proteins and RNAs. Learn More...

Peking University

Peking University is a comprehensive and National key university. The campus, known as "Yan Yuan"-- the gardens of Yan, is situated at the northeast of the Haidian District at the western suburbs of Beijing. It stands near the Yuan Ming Gardens and the Summer Palace.

The university has made an effective combination of the research on important scientific issues with the training of personnel with high level specialized knowledge and professional skill as demanded by the country's socialist modernization. It strives not only for the simultaneous improvements in teaching and research work, but also for the promotion of interaction and mutual promotion among various subjects.

Peking University has become a center for teaching and research and a university of the new type, consisting of diverse branches of learning such as pure and applied sciences, social sciences and the humanities, and sciences of management and education. Its aim is to rank among the world's best universities at the beginning of the next century. Learn More...

Road to Jamboree

Acknowledgement


  

 

Notebook Parts The Model The Project The Team Home

Retrieved from "http://2009.igem.org/Team:PKU_Beijing"