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Team:PKU Beijing/Project/AND Gate 1/Design
From 2009.igem.org
Mechanism
Many AND Gate have been designed and constructed in the past years. In our project, We adopted the design of Chris Voigt[1].
fig1. The mechanism of the AND Gate
Fig.1 illustrates the mechanism of the design. One of the inputs of the and gate leads to the expression of T7ptag mRNA which is a T7 polymerase coding sequence with amber mutations inside. The other input of the And gate leads to the expression of supD tRNA, which is a amber mutation suppressor. The concurrence of the mRNA and the tRNA can make functional T7 polymerase, which can activate the downstream T7 promoter.
