Team:TUDelft/27 August 2009

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27 August 2009

Saeed

The overnight colonies were used for miniprep. !Za, !Zb, !Zg and !Zh had the highest DNA concentrations. These were downstream cut and checked on 1% agarose gel for fragment size. Fragment should be 1934 bp.

Gelsaeed27-082009 !Z.wiki.jpg

lane Part Expected Plasmid Size Status
1 [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder]
2  !Za 1934 no band
3  !Zb 1934 ok
4  !Zg 1934 ok
5  !Zh 1934 ok
6 [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder]

!Zb, !Zg and !Zh seem to be ok. One of these shoudl be used for sequencing.



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