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Team:TUDelft/Project
From 2009.igem.org
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*Construction and characterization of an inducible or self regulated auto-destructive plasmid | *Construction and characterization of an inducible or self regulated auto-destructive plasmid | ||
*Tune the differential expression of (at least) two genes within the same plasmid | *Tune the differential expression of (at least) two genes within the same plasmid | ||
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*Cell synchronization | *Cell synchronization | ||
*Selectable marker recycling | *Selectable marker recycling | ||
Revision as of 10:55, 29 May 2009
Page is under construction
Overall project
Our abstract
Finally TUDelft team has a project!!!
Self-destructive plasmid
Goals:
- Construction and characterization of an inducible or self regulated auto-destructive plasmid
- Tune the differential expression of (at least) two genes within the same plasmid
- Construction of a plasmid which contains the gene of a specific restriction enzyme that, after expression, will destroy the plasmid
- Construction of a plasmid with enough and specific restriction sites which leads to its destruction under restriction enzyme treatment
- Clone and incorporation of the plasmid
Why?
- To test one condition and return to the original state without kill the cell or change any of its previous genetic and physiological characteristics
- To generate a pulse in the expression of a certain gene
- To control the amplitude of the pulse created by the plasmid
- Because it is cool!!!
How?
Not known yet :D
Applications:
- Cell synchronization
- Selectable marker recycling
- Metabolic control analysis
- Recombination
- …
Project Details
The Experiments
Results
