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Team:TUDelft/Project
From 2009.igem.org
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<br> | <br> | ||
| - | + | ='''Project Overview'''= | |
| - | + | =='''Our abstract'''== | |
| - | ''Our abstract'' | + | <br> |
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Finally TUDelft team has a project!!! | Finally TUDelft team has a project!!! | ||
| - | + | ===Self-destructive plasmid=== | |
| - | =Self-destructive plasmid= | + | |
[[Image:TUDelft_logo.png|50px|right|frame]] | [[Image:TUDelft_logo.png|50px|right|frame]] | ||
Goals: | Goals: | ||
Revision as of 20:18, 5 June 2009
Page is under construction
Project Overview
Our abstract
Finally TUDelft team has a project!!!
Self-destructive plasmid
Goals:
- Construction and characterization of an inducible or self regulated auto-destructive plasmid
- Tune the differential expression of (at least) two genes within the same plasmid
- Construction of a plasmid which contains the gene of a specific restriction enzyme that, after expression, will destroy the plasmid
- Construction of a plasmid with enough and specific restriction sites which leads to its destruction under restriction enzyme treatment
- Clone and incorporation of the plasmid
Why?
- To test one condition and return to the original state without kill the cell or change any of its previous genetic and physiological characteristics
- To generate a pulse in the expression of a certain gene
- To control the amplitude of the pulse created by the plasmid
- Because it is cool!!!
How?
Not known yet :D
Applications:
- Cell synchronization
- Selectable marker recycling
- Metabolic control analysis
- Recombination
- …
Project Details
The Experiments
Results
