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Team:TUDelft/Project
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Overall project
Our abstract
Finally TUDelft team has a project!!!
Self-destructive plasmid
Goals
- Construction and characterization of an inducible or self regulated auto-destructive plasmid - Tune the differential expression of (at least) two genes within the same plasmid - Construction of a plasmid which contains the gene of a specific restriction enzyme that, after expression, will destroy the plasmid - Construction of a plasmid with enough and specific restriction sites which leads to its destruction under restriction enzyme treatment - Clone and incorporation of the plasmid
Why?
- To test one condition and return to the original state without kill the cell or change any of its previous genetic and physiological characteristics - To generate a pulse in the expression of a certain gene - To control the amplitude of the pulse created by the plasmid - Because it is cool!!!
How?
Not known yet :D
Applications
- Cell synchronization - Selectable marker recycling - Metabolic control analysis - Recombination - …
Project Details
The Experiments
Results
