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Team:Todai-Tokyo/Protocols/PCR/Enzymes
From 2009.igem.org
(Difference between revisions)
(New page: {{:Team:Todai-Tokyo/Template}} == Taq Polymerases == === Pfu Ultra === Unless otherwise stated, we use this Taq * 0.2µl 100µM 5’primer * 0.2µl 100µM 3’primer * 1.6µl 2....) |
(→Pfu Ultra) |
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=== Pfu Ultra === | === Pfu Ultra === | ||
| - | Unless otherwise stated, we use this | + | Unless otherwise stated, we use this polymerase |
| - | + | ||
* 0.2µl 100µM 5’primer | * 0.2µl 100µM 5’primer | ||
* 0.2µl 100µM 3’primer | * 0.2µl 100µM 3’primer | ||
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* 0.05µl DNA （to a final concentration of 5-30ng/20ul） | * 0.05µl DNA （to a final concentration of 5-30ng/20ul） | ||
* 0.5µl Pfu Ultra2 | * 0.5µl Pfu Ultra2 | ||
| - | |||
=== Ex Taq === | === Ex Taq === | ||
Latest revision as of 10:45, 20 October 2009
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Contents |
Taq Polymerases
Pfu Ultra
Unless otherwise stated, we use this polymerase
- 0.2µl 100µM 5’primer
- 0.2µl 100µM 3’primer
- 1.6µl 2.5mM dNTP
- 2µl 10 x Pfu Ultra 2 buffer
- 15.45µl MilliQ
- 0.05µl DNA （to a final concentration of 5-30ng/20ul）
- 0.5µl Pfu Ultra2
Ex Taq
- 0.2µl 100µM 5’primer
- 0.2µl 100µM 3’primer
- 1.6µl 2.5mM dNTP
- 2µl 10 x Pfu Ultra 2 buffer
- Xµl MilliQ（up to 20ul）
- Xµl DNA（to a final concentration of 200ng/20ul）
- 0.2µl Ex-Taq
KOD plus
- 5μl 10 × PCR Buffer for KOD-Plus
- 5μl 2mM dNTPs
- 2μl 25mM MgSO4
- 1.5μl 10μM primer 1
- 1.5μl 10μM primer 2
- >1 μl template DNA
- Genomic DNA 10~200ng/50μl
- Plasmid DNA 1~50ng/50μl
- 1 μl KOD-Plus
- MilliQ to 50μl
