Team:TorontoMaRSDiscovery/24 September 2009

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September 24, 2009

  1. Made a new batch of chemically competent cells
    • poured cells into 50ml falcon tubes
    • 350ul aliquots + 350ul 40% glycerol
  2. Started 12E overnights from transfections done yesterday
    • Named: 1-5, a-e, but these have been used already, hence 1-5 were renamed 6-10, a-e renamed f-j
  3. Transformed ligation from Monday and negative control into new cells and plated on new K plates
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