Team:TorontoMaRSDiscovery/4 September 2009
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September 4, 2009
- Miniprepped overnight cultures
- stocked Amp, K1, K2, and Enc+5 (2), (3), (4)
- Digested Enc+5 (1)-(4), K1,2, BB7
- Transfected 1+2+3+2 into DH-5 cells (50ul)
- left on ice for 2.5h after adding ligation product
- incubated on shaker for about 2h
- Started overnight of C plasmid (ccdb)