Team:TorontoMaRSDiscovery/9 October 2009

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October 9, 2009

  1. Miniprepped EncY overnight and digested with E,P
  2. Ran digest on a gel
    • Enc part confirmed
  3. Transfected Enc+5 ligations from Sunday/Monday into competent cells from Invitrogen
    • Used NEB Hight Efficiency Transformation Protocol C2987
      • did one 10-fold dilution
      • transformed 5ul of DNA
      • spread 100ul of cells on plate
      • shaker/incubator speed 232rpm
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