Team:TorontoMaRSDiscovery/Project

From 2009.igem.org

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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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!align="center"|[[Team:TorontoMaRSDiscovery|Home]]
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!align="center"|[[Team:TorontoMaRSDiscovery/Team|The Team]]
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!align="center"|[[Team:TorontoMaRSDiscovery/Project|The Project]]
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''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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!align="center"|[[Team:TorontoMaRSDiscovery/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:TorontoMaRSDiscovery/Modeling|Modeling]]
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!align="center"|[[Team:TorontoMaRSDiscovery/Notebook|Notebook]]
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|align="center"|[[Team:TorontoMaRSDiscovery | Team Example 2]]
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===Engineering bacterial micro-compartments to investigate metabolic channeling and its potential uses in biotechnological applications===
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== '''Overall project''' ==
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==Background==
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The University of Toronto iGem team year is trying to create a bacteria that exhibits substrate channeling using nano-compartments. Our experiments are designed to characterize the nano-compartment for future use and establish a proof of concept for the use of nanocompartments in the iGem competition
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It is well established that genes and their products do not operate in isolation but rather form parts of integrated biochemical pathways. There is increasing evidence that in many pathways, individual components exhibit varying degrees of spatial organization ranging from sub-cellular compartmentalization to the formation of discrete complexes.
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For metabolic processes, the co-localization of enzymatic components has been shown to promote the transfer of substrates between consecutive reactions in a process termed “channeling”1. Metabolic channeling is defined as the process in which the intermediate produced by one enzyme is transferred to the next enzyme without complete mixing in the bulk phase12. Channeling results in the efficient translocation of substrates between enzymes and has been proposed to result in the following benefits: 1) Optimization of catalytic efficiency by decreasing transit time for intermediates; 2) Relief of the effects of product inhibition; 3) Protection from the creation of potentially toxic or unstable intermediates; and 4) Regulation of substrate flux through mediating pathway cross-talk 1. Potential applications range from the production of valuable compounds such as therapeutic molecules and biofuels2 to the degradation of toxic wastes3.
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We propose a novel interdisciplinary project to systematically investigate how nature implements metabolic channeling and how this knowledge may be exploited for biotechnological applications.
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==Experimental Approach==
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'''Objectives
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#Design, construct and characterize a micro-compartment expression system in E. coli.
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== Project Details==
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#Demonstrate in vivo assembly of the expressed micro-compartments.
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#Target a fluorescent marker (eCFP) to the micro-compartment.
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#Identify and prioritize candidate enzyme pairs for channeling.
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#Apply channeling to selected enzyme pairs.
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=== Part 2 ===
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=== The Experiments ===
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=== Part 3 ===
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== Results ==
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<!--- The Mission, Experiments --->
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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!align="center"|[[Team:TorontoMaRSDiscovery|Home]]
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!align="center"|[[Team:TorontoMaRSDiscovery/Team|The Team]]
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!align="center"|[[Team:TorontoMaRSDiscovery/Project|The Project]]
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!align="center"|[[Team:TorontoMaRSDiscovery/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:TorontoMaRSDiscovery/Modeling|Modeling]]
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!align="center"|[[Team:TorontoMaRSDiscovery/Notebook|Notebook]]
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(''Or you can choose different headings. But you must have a team page, a project page, and a notebook page.'')
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Revision as of 03:04, 21 September 2009

Top(nolink).png

Home The Team The Project Parts Submitted to the Registry Modeling Notebook


Engineering bacterial micro-compartments to investigate metabolic channeling and its potential uses in biotechnological applications

Background

It is well established that genes and their products do not operate in isolation but rather form parts of integrated biochemical pathways. There is increasing evidence that in many pathways, individual components exhibit varying degrees of spatial organization ranging from sub-cellular compartmentalization to the formation of discrete complexes.

For metabolic processes, the co-localization of enzymatic components has been shown to promote the transfer of substrates between consecutive reactions in a process termed “channeling”1. Metabolic channeling is defined as the process in which the intermediate produced by one enzyme is transferred to the next enzyme without complete mixing in the bulk phase12. Channeling results in the efficient translocation of substrates between enzymes and has been proposed to result in the following benefits: 1) Optimization of catalytic efficiency by decreasing transit time for intermediates; 2) Relief of the effects of product inhibition; 3) Protection from the creation of potentially toxic or unstable intermediates; and 4) Regulation of substrate flux through mediating pathway cross-talk 1. Potential applications range from the production of valuable compounds such as therapeutic molecules and biofuels2 to the degradation of toxic wastes3.

We propose a novel interdisciplinary project to systematically investigate how nature implements metabolic channeling and how this knowledge may be exploited for biotechnological applications.

Experimental Approach

Objectives

  1. Design, construct and characterize a micro-compartment expression system in E. coli.
  2. Demonstrate in vivo assembly of the expressed micro-compartments.
  3. Target a fluorescent marker (eCFP) to the micro-compartment.
  4. Identify and prioritize candidate enzyme pairs for channeling.
  5. Apply channeling to selected enzyme pairs.
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