Team:UAB-Barcelona/Primersn

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Encap raro.jpg Documento sin título

These were the primers we ordered for the nitrite:

• Pnir: EcoR1-Not-Xba— nirk – Spe

Reverse:

                S
    GTCAACGT ACTAGT CTGCACGAAAGACCTTTAAATAC     Temperature annealing = 62 ºC

Forward:

                 E      N       X
     CAGTAGTA GAATTC GCGGCCGC TCTAGA GTTGCGCGAAATACCGTAAC   Temperature annealing= 60 ºC