Team:UAB-Barcelona/ext

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We used in both cases Wizard Genomic DNA Purification Kit (PROTOCOL).

Extraction of N. europaea DNA

To verify that we had enough concentration of DNA to makethe PCR (approximately 100 ng of DNA) we measured the concentration and purity of the DNA (there is recommended that the ratio 260/280 should be major that 1.8) with the Nanodrop. Furthermore, we verified the integritied of the DNA by electrophoresis.

Quantification of Nitrosomonas  genomic DNA by Nanodrop
[DNA] (ng/microl)               Relation Abs 260/280
74.1	                                 1.92
104.2	                                 1.92
241.0	                                 1.95
284.6	                                 1.87

Agarose gel to verify the integrity of the Nitrosomonas europea genomic DNA.

From left side to right: Gene ruler DNA Ladder; Nitrosomonas europea genomic DNA.

Extraction of E.coli DNA

Quantification of E. coli  genomic DNA by Nanodrop
[DNA] (ng/microl)	Relation Abs 260/280
885	                      2.16
1507	                      2.05

Agarose gel to verify the integrity of the E. coli genomic DNA.