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Team:UC Davis/wiring pH sensor
From 2009.igem.org
(Difference between revisions)
| Line 48: | Line 48: | ||
Th pH sensor: </span></big><br> | Th pH sensor: </span></big><br> | ||
ChvG/ChvI is a | ChvG/ChvI is a | ||
| - | two component system | + | two-component system and a suspected pH sensor. ChvG is the histidine kinase and ChvI is its |
| - | suspected pH sensor. ChvG is the histidine kinase and ChvI is its | + | cognate response regulator(2). Together they are believed to sense |
| - | cognate response regulator (2). Together they are believed to sense | + | extracellular pH between 5.5 to 7 and relay this signal to regulate |
| - | extracellular pH between 5.5 to 7 and | + | the expression of key virulence-related genes(2).<br> |
| - | the expression of key virulence related genes | + | |
In our design, we used this two-component system from <i>Agrobacterium | In our design, we used this two-component system from <i>Agrobacterium | ||
| - | tumefaciens</i> | + | tumefaciens</i> to regulate the expression of select target genes.<br> |
<hr style="width: 100%; height: 2px;"><big><span | <hr style="width: 100%; height: 2px;"><big><span | ||
style="text-decoration: underline; font-weight: bold;">Possibility of | style="text-decoration: underline; font-weight: bold;">Possibility of | ||
| - | Cross Talk:</span></big><br> | + | Cross-Talk:</span></big><br> |
Bacteria have | Bacteria have | ||
| - | numerous related | + | numerous related two-component systems based on kinase/response-regulation(5). For instance, the <i>A. tumefaciens</i> |
| - | (5). For instance, the <i> | + | |
genome is known to contain at least 25 two-component pathways | genome is known to contain at least 25 two-component pathways | ||
| - | (2).Therefore, there are possibilities of | + | (2). Therefore, there are possibilities of cross-talk between some genes |
| - | present in <i>E. coli</i> that have the same mechanism as ChvI/ChvG | + | present in <i>E. coli</i> that have the same mechanism as the ChvI/ChvG |
system. In fact, there is evidence supporting that ChvI can complement | system. In fact, there is evidence supporting that ChvI can complement | ||
a PhoB deletion in <i>E. coli</i>. (3) Therefore it is highly likely | a PhoB deletion in <i>E. coli</i>. (3) Therefore it is highly likely | ||
| - | that there is a possibility of cross talk between our new sensory | + | that there is a possibility of cross-talk between our new sensory |
systems elements, present in <i>E. coli</i>. | systems elements, present in <i>E. coli</i>. | ||
Many protein-protein interactions are not fully understood (5), which | Many protein-protein interactions are not fully understood (5), which | ||
Revision as of 02:37, 22 October 2009
Wiring the pH
sensor into our system:
Th pH sensor:
ChvG/ChvI is a two-component system and a suspected pH sensor. ChvG is the histidine kinase and ChvI is its cognate response regulator(2). Together they are believed to sense extracellular pH between 5.5 to 7 and relay this signal to regulate the expression of key virulence-related genes(2).
In our design, we used this two-component system from Agrobacterium tumefaciens to regulate the expression of select target genes.
Possibility of Cross-Talk:
Bacteria have numerous related two-component systems based on kinase/response-regulation(5). For instance, the A. tumefaciens genome is known to contain at least 25 two-component pathways (2). Therefore, there are possibilities of cross-talk between some genes present in E. coli that have the same mechanism as the ChvI/ChvG system. In fact, there is evidence supporting that ChvI can complement a PhoB deletion in E. coli. (3) Therefore it is highly likely that there is a possibility of cross-talk between our new sensory systems elements, present in E. coli. Many protein-protein interactions are not fully understood (5), which makes this project more challenging but very interesting.
Click on the specific part or
interaction for more information.
Parts: ChvG, ChvI, PhoR, PhoB
Interactions: ChvG---> ChvI , PhoR---> ChvI , PhoR--->PhoB ,ChvI--->PhoA
Th pH sensor:
ChvG/ChvI is a two-component system and a suspected pH sensor. ChvG is the histidine kinase and ChvI is its cognate response regulator(2). Together they are believed to sense extracellular pH between 5.5 to 7 and relay this signal to regulate the expression of key virulence-related genes(2).
In our design, we used this two-component system from Agrobacterium tumefaciens to regulate the expression of select target genes.
Possibility of Cross-Talk:
Bacteria have numerous related two-component systems based on kinase/response-regulation(5). For instance, the A. tumefaciens genome is known to contain at least 25 two-component pathways (2). Therefore, there are possibilities of cross-talk between some genes present in E. coli that have the same mechanism as the ChvI/ChvG system. In fact, there is evidence supporting that ChvI can complement a PhoB deletion in E. coli. (3) Therefore it is highly likely that there is a possibility of cross-talk between our new sensory systems elements, present in E. coli. Many protein-protein interactions are not fully understood (5), which makes this project more challenging but very interesting.
Parts: ChvG, ChvI, PhoR, PhoB
Interactions: ChvG---> ChvI , PhoR---> ChvI , PhoR--->PhoB ,ChvI--->PhoA
