"
Team:UC Davis/wiring pH sensor
From 2009.igem.org
(Difference between revisions)
| Line 38: | Line 38: | ||
style="border: 0px solid ; width: 83px; height: 37px;"></a></big></big></big></big></span></b><b | style="border: 0px solid ; width: 83px; height: 37px;"></a></big></big></big></big></span></b><b | ||
style="color: rgb(0, 0, 0);"><span | style="color: rgb(0, 0, 0);"><span | ||
| - | style="font-size: 13.5pt; font-family: "Times New Roman","serif";" | + | style="font-size: 13.5pt; font-family: "Times New Roman","serif";"></span><br> |
</b> | </b> | ||
<hr style="width: 100%; height: 2px;"> | <hr style="width: 100%; height: 2px;"> | ||
| Line 69: | Line 69: | ||
makes this project more challenging but very interesting. | makes this project more challenging but very interesting. | ||
<br> | <br> | ||
| - | <hr style="width: 100%; height: 2px;"><br> | + | <hr style="width: 100%; height: 2px;"> |
| - | <br> | + | <div style="text-align: center;"><span style="font-style: italic;"></span><br> |
| - | <big><span style="font-weight: bold; text-decoration: underline;"> </span></big> | + | <span style="font-style: italic;"></span></div> |
| + | <span style="font-style: italic;">Click on the specific part or | ||
| + | interaction for more information.<br> | ||
| + | Parts: <a href="https://2009.igem.org/Team:UC_Davis/Project1/ChvG.html">ChvG</a>,<a | ||
| + | href="https://2009.igem.org/Team:UC_Davis/ChvI1">ChvI</a>,<a | ||
| + | href="https://2009.igem.org/Team:UC_Davis/PhoR1">PhoR</a>,<a | ||
| + | href="https://2009.igem.org/Team:UC_Davis/PhoB1">PhoB</a><br> | ||
| + | interactions: <a href="https://2009.igem.org/Team:UC_Davis/ChvG_ChvI">ChvG---> | ||
| + | ChvI</a> , <a href="https://2009.igem.org/Team:UC_Davis/PhoR_ChvI1">PhoR---> | ||
| + | ChvI </a>, <a href="https://2009.igem.org/Team:UC_Davis/PhoR_PhoB">PhoR--->PhoB</a> | ||
| + | ,<a href="https://2009.igem.org/Team:UC_Davis/ChvI_PhoA">ChvI--->PhoA<br> | ||
| + | </a><br> | ||
| + | </span><big><span style="font-weight: bold; text-decoration: underline;"> | ||
| + | </span></big> | ||
<p class="MsoNormal" | <p class="MsoNormal" | ||
| - | style="background: white none repeat scroll 0%; text-indent: 0.5in; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial;"><b><span | + | style="background: white none repeat scroll 0% 50%; text-indent: 0.5in; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial;"><b><span |
style="font-size: 14pt; font-family: "Times New Roman","serif"; color: black;"><o:p></o:p></span></b></p> | style="font-size: 14pt; font-family: "Times New Roman","serif"; color: black;"><o:p></o:p></span></b></p> | ||
</div> | </div> | ||
Revision as of 04:08, 8 October 2009
Wiring the pH
sensor into our system:
Th pH sensor:
ChvG is a suspected pH sensor and histidine kinase and ChvI is its cognate response regulator (2). Together they are believed to sense extracellular pH between 5.5 to 7 and relaying this signal to regulate the expression of key virulence related genes. (2)
In our design, we used this two-component system from Agrobacterium tumefaciens, to regulate the expression of select target genes.
Possibility of Cross Talk:
Bacteria have numerous related kinase/response regulated based, two component systems (5). For instance, the Agrobacterium tumefaciens genome is known to contain at least 25 two-component pathways (2).Therefore, there are possibilities of crosstalk between some genes present in E. coli that have the same mechanism as ChvI/ChvG system. In fact, there is evidence supporting that ChvI can complement a PhoB deletion in E. coli. (3) Therefore it is highly likely that there is a possibility of cross talk between our new sensory systems elements, present in E. coli. Many protein-protein interactions are not fully understood (5), which makes this project more challenging but very interesting.
Click on the specific part or
interaction for more information.
Parts: ChvG,ChvI,PhoR,PhoB
interactions: ChvG---> ChvI , PhoR---> ChvI , PhoR--->PhoB ,ChvI--->PhoA
Th pH sensor:
ChvG is a suspected pH sensor and histidine kinase and ChvI is its cognate response regulator (2). Together they are believed to sense extracellular pH between 5.5 to 7 and relaying this signal to regulate the expression of key virulence related genes. (2)
In our design, we used this two-component system from Agrobacterium tumefaciens, to regulate the expression of select target genes.
Possibility of Cross Talk:
Bacteria have numerous related kinase/response regulated based, two component systems (5). For instance, the Agrobacterium tumefaciens genome is known to contain at least 25 two-component pathways (2).Therefore, there are possibilities of crosstalk between some genes present in E. coli that have the same mechanism as ChvI/ChvG system. In fact, there is evidence supporting that ChvI can complement a PhoB deletion in E. coli. (3) Therefore it is highly likely that there is a possibility of cross talk between our new sensory systems elements, present in E. coli. Many protein-protein interactions are not fully understood (5), which makes this project more challenging but very interesting.
Parts: ChvG,ChvI,PhoR,PhoB
interactions: ChvG---> ChvI , PhoR---> ChvI , PhoR--->PhoB ,ChvI--->PhoA
