Team:UNIPV-Pavia/Methods Materials/Glycerol stock

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== Gycerol stock ==  
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== '''Gycerol stock''' ==  
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''(estimated time: 15 min + freezing time)''
''(estimated time: 15 min + freezing time)''

Revision as of 16:20, 22 June 2009


Gycerol stock


(estimated time: 15 min + freezing time)

Materials needed:

  • sample of overnight incubated bacteria
  • 80% glycerol


  • Mix 750 ul from the 5 ml sample of overnight incubated bacteria with 250 ul of 80% glycerol
  • Leave at -20°C for one day
  • Move to -80°C the day after