Team:UNIPV-Pavia/Methods Materials/Glycerol stock

From 2009.igem.org

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(→Glycerol stocks)
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*Mix 750 ul from the 5 ml sample of overnight incubated bacteria with 250 ul of 80% glycerol
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*Mix 750 ul from the 5 ml sample of the incubated bacteria with 250 ul of 80% glycerol
*Leave at -20°C for one day
*Leave at -20°C for one day
*Move to -80°C the day after
*Move to -80°C the day after
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*'''o/n incubated bacteria'''
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*'''o/n or in exp phase bacterial culture'''
*'''80% glycerol'''
*'''80% glycerol'''
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Revision as of 19:48, 1 July 2009

EthanolPVanimation.gif



Protocols


Glycerol stocks

(estimated time: 15 min + freezing time)

  • Mix 750 ul from the 5 ml sample of the incubated bacteria with 250 ul of 80% glycerol
  • Leave at -20°C for one day
  • Move to -80°C the day after










  • o/n or in exp phase bacterial culture
  • 80% glycerol




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