Team:UNIPV-Pavia/Methods Materials/LB

From 2009.igem.org

(Difference between revisions)
(→LB medium)
(→LB medium)
 
(6 intermediate revisions not shown)
Line 1: Line 1:
{{UNIPV-Pavia/StyleCss}}
{{UNIPV-Pavia/StyleCss}}
<html>
<html>
-
<head>
+
 
-
<link rel="stylesheet" type="text/css" href="http://bioinfo.unipv.it/igem/templates/SpryAssets/SpryMenuBar.js" title="Stile"/>
+
-
</head>
+
-
<body>
+
<table width="100%">
<table width="100%">
   <tr>
   <tr>
<td align="center">
<td align="center">
-
<img width="100%" height="180px" src="https://static.igem.org/mediawiki/2009/c/c2/UNIPV_title_MandM.jpg" ></img>
+
<img width="100%" height="180px" src="https://static.igem.org/mediawiki/2009/d/de/UNIPV_title_M%26M.jpg" ></img>
</td>
</td>
   </tr>
   </tr>
</table>
</table>
-
</body>
+
 
</html>
</html>
Line 25: Line 22:
     <font face="Pristina" size="50" ><b>Protocols</b></font>
     <font face="Pristina" size="50" ><b>Protocols</b></font>
     </td>
     </td>
 +
    <td width="50px"></td>
</html>
</html>
   </tr>
   </tr>
Line 54: Line 52:
*Always check for contaminations before using!
*Always check for contaminations before using!
<br><br><br><br><br><br><br><br>
<br><br><br><br><br><br><br><br>
-
<br></td><td class='beuta' align='center' valign='bottom' width='300px'>
+
<br></td><td align='center' valign='top' width='300px'>
-
<div align='center'>
+
 
<table border='1'>
<table border='1'>
 +
<tr bgcolor='yellow'><td>INGREDIENTS:
 +
</td></tr>
<tr><td>
<tr><td>
<font class='label'>
<font class='label'>
Line 65: Line 65:
'''ddH2O'''<hr color='white' width='70%'>
'''ddH2O'''<hr color='white' width='70%'>
'''Agar''' </font>
'''Agar''' </font>
-
</td></tr></table></div><br><br><br><br>
+
</td></tr></table><br><br><br><br>
</td></tr></table>
</td></tr></table>

Latest revision as of 10:25, 21 October 2009

EthanolPVanimation.gif



Protocols



LB medium

(Estimated time: 10 min + 4 hours of autoclavation and cooling)

  • For a final volume of 1 l, put:
    • 10 g NaCl
    • 10 g BactoTryptone
    • 5 g Yeast extract
    • 15 g Agar (only for LB plates)
  • into 1 l of ddH2O.
  • Autoclave the solution.
  • Let it cool to ~40-45°C
  • Add the proper antibiotic if needed.
  • ONLY FOR PLATES:
    • Pour an homogenous layer of agar LB into Petri plates under the hood.
    • Let agar LB polymerase.
  • Cover and store at +4°C.
  • Always check for contaminations before using!










INGREDIENTS:

Clean bottle or flask
NaCl
BactoTryptone
Yeast extract
ddH2O

Agar





Retrieved from "http://2009.igem.org/Team:UNIPV-Pavia/Methods_Materials/LB"